摘要
目的:旨在建立一种快速、简便的针对磺胺二甲基嘧啶残留的检测方法。方法:应用竞争抑制免疫层析原理,研制磺胺二甲基嘧啶胶体金试纸条。结果:该试纸条检测限为10ng/mL、检测时间5min,与其他磺胺类药物交叉反应小,可用于多种食品样本的检测,且样本检测限为100μg/kg。结论:该方法操作简便、灵敏度高且特异性强,可实现对食品样品中磺胺二甲基嘧啶残留的快速检测。
Objective： To establish a rapid and convenient detection method for sulfadimidine residue in food. Methods： The colloidal gold strip for specific sulfadimidine was developed successfully on the basis of competitive inhibition immunochromatography mechanism. Results： The detection limit of the colloidal gold strip was 10 ng/mL for sulfadimidine within less than 5 min, which also had low cross-reactivity with other sulfonamides. The colloidal gold strip could be used in rapid detection of sulfadimidine residue in many kinds of food samples with detection limit of 100 μg/kg. Conclusion： This method is characteristics of simplicity, high sensitivity and specificity to achieve the rapid detection of sulfadimidine residue in food.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2010年第24期366-369,共4页
Food Science
基金
教育部"长江学者和创新团队发展计划"资助项目(IRT0540)
南昌市重大产业化项目(2009)
