摘要
目的观察大孔径碳纳米管(SWNTs)做为载体以增加顺铂抗肿瘤活性的模式及机制。方法用毛细现象,将碘化丙啶(PI)和顺铂装载进入纳米管管腔,内载顺铂进入宫颈癌细胞,并在细胞内释放顺铂;用荧光分光光度计及紫外分光光度计,观察PI及顺铂在细胞外和细胞内释放模式,并通过流式细胞技术及MTT法,检测顺铂对宫颈癌细胞增殖活性的抑制。结果顺铂在碳纳米管的运载下,可较易进入细胞内,从而使细胞内药物浓度增加,其模式与PI的转载模式相同,且在顺铂浓度较低时,就可对Hela细胞产生明显的抑制作用。结论 SWNTs内载抗癌药物可逆转肿瘤耐药,增加药物抗肿瘤作用。
Objective To study the extracellular as well as the intracellular drug release mode,the drug delivery ability,and the anticancer potential of single-walled carbon nanotubes（SWNTs）with larger diameters（20-50 nm）loaded with cisplatin or propidium iodide（PI）in cervical cancer cells.Methods Determination of extracellular and intracellar PI and cisplatin release mode of drug-loaded SWNTs by UV spectrophotometer and fluorospectrophotometer.To observe the cytotoxic effect of cisplatin-loaded SWNTs on the proliferation of Hela cells by MTT assay and flow cytometry detection.Results Our findings was demonstrated that SWNTs with larger diameters（20-50 nm）has good membrane penetration and drug delivery abilities.Cisplatin-loaded SWNTs has a similar extracellular drug release mode to PI-loaded SWNTs,and cisplatin-loaded SWNTs can enhance the anticancer effects of cisplatin in cervical cancer cells in a concentration-dependent manner.Conclusion Our study was suggested that SWNTs with larger diameters（20-50 nm）is a good carrier for fluorescent reagents and therapeutic agents,and cisplatin-loaded SWNTs with larger diameters（20-50 nm）is a promising anti-cancer agent for cervical cancers.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2010年第12期920-922,953,共4页
The Chinese Journal of Clinical Pharmacology
关键词
大孔径纳米管
顺铂
HELA细胞
增殖活性
single-walled carbon nanotubes with larger diameters
cisplatin
cervical cancer cell
proliferation
