摘要
目的 评价根动脉注射氯胺酮对犬脊髓的影响.方法 健康成年犬20只,雌雄不拘,体重12～18 kg-随机分为2组（n=10）：对照组（C组）和氯胺酮组（K组）.经肋间后动脉,向根动脉方向置管至根动脉分支处,C组注射生理盐水2 ml,K组注射氯胺酮200 mg（用生理盐水稀释至2 ml）.药物注射完毕后3 h,取根动脉相对应的脊髓节段,切片,分别行HE染色和尼氏染色,光镜下观察脊髓组织病理学结果,计数阴性神经元（不含有尼氏小体的神经元）;采用免疫组化法测定神经元特异性烯醇化酶、S100β蛋白和Tau蛋白的表达水平.结果 两组脊髓神经元形态正常,尼氏小体丰富,均未见阴性神经元.两组脊髓神经元特异性烯醇化酶、S100β蛋白和Tau蛋白的表达比较差异无统计学意义（P＞0.05）.结论根动脉注射氯胺酮不会导致犬脊髓损伤.
Objective To investigate the effect of ketamine injected via the radicular arteries on spinal cord. Methods Twenty healthy mongrel dogs of both sexes weighing 12-18 kg were randomly divided into 2 groups （ n = 10 each）： control group （group C） and ketamine group （group K）. The animals were anesthetized with intravenous pentobarbital 30-35 mg/kg, fentanyl 50-100 μg and vecuronium 0.2 mg/kg and maintained with propofol ically ventilated after tracheal intubation. A catheter was inserted into T8 poster intercostal artery and advanced toward the opening of radicular artery which supplies the spinal cord. Ketamine 100 mg （in 2 ml of normal saline）was injected via the catheter in group K. Three hours after ketamine administration, the animals were sacrificed. A 1.5 cm long segment of spinal cord at the level of T8 was removed for microscopic examination and determination of the expression of NSE, S100β and Tau protein by immuno-histochemistry. Results There was no significant difference in the number of Nissl＇ s staining-negative neuronal cells and the expression of NSE, S100β and Tau protein in the spinal cord between the 2 groups （ P 〉 0.05 ）. Conclusion Ketamine injected via the radicular arteries does not induce spinal cord injury.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2010年第10期1201-1203,共3页
Chinese Journal of Anesthesiology
关键词
氯胺酮
注射
动脉内
脊髓
Ketamine
Injections, intra-arterial
Spinal cord
