摘要
采用HPLC-UV-ELSD串联技术,建立西洋参药材皂苷类成分HPLC-UV-ELSD特征图谱的质量控制方法。色谱柱为Agilent Extend-C18(250 mm×4.6 mm,5μm),乙腈-水二元梯度洗脱模式,流速为1.0 mL·min-1,检测波长为203 nm,漂移管温度为106.5℃,空气流速为2.9 L·min-1,对20批西洋参药材进行HPLC-UV-ELSD测定,采用聚类分析和主成分分析对特征图谱数据进行了模式识别研究,并指认了人参皂苷Rg1、Re、Rb1、Rc、Rb2、Rb3、Rd及拟人参皂苷F11等8个色谱峰。所建立的西洋参皂苷类成分特征图谱特征性和专属性强,方法快速、简便、可靠,可用于西洋参质量控制及综合评价。
The paper is to report the establishment of a method of characteristic figure analysis for the quality control of Panacis Quinquefolii Radix.Application of HPLC-UV-ELSD techniques was connected in series and applied.The separation was carried out on the Agilent Extend-C18（250 mm×4.6 mm,5 μm） column.The mobile phase consisted of water and acetonitrile with gradient elution.The flow rate was 1.0 mL·min-1 and the wavelength of measurement was 203 nm.The temperature of drift tube was maintained at 106.5 ℃ and the flow rate of air was set at 2.9 L.min-1.Twenty batches of the Panacis Quinquefolii Radix were determined.Hierarchical cluster analysis（HCA） and principal component analysis（PCA） were applied to study on the HPLC characteristic figure and chemical pattern recognition.The HPLC-UV and HPLC-ELSD characteristic figure of Panacis Quinquefolii Radix was developed,the ginsenosides Rg1,Re,Rb1,Rc,Rb2,Rb3,Rd and the pseudoginsenoside F11 were identified.This method is accurate and reliable,and it can be used to control the quality of the Panacis Quinquefolii Radix.
出处
《药学学报》
CAS
CSCD
北大核心
2011年第2期198-202,共5页
Acta Pharmaceutica Sinica
基金
国家科技支撑计划项目(2006BAI09B02)
中国博士后科学基金(20080440329)
关键词
西洋参
皂苷类成分
HPLC-UV-ELSD
特征图谱
聚类分析
主成分分析
Panacis Quinquefolii Radix
ginsenosides
HPLC-UV-ELSD
chromatographic characteristic figure
hierarchical clustering analysis
principle component analysis
