摘要
目的 了解肺癌患者体内自身抗体阳性率、细胞内定位及荧光模式特点,分析自身抗体谱与肺癌的相关性.方法 收集36例病理诊断为原发性肺癌患者的血清,其中16例尚未行手术及化疗药物治疗,20例已行手术切除且正处于化疗阶段;另外收集47例正常人血清用于对照.采用间接免疫荧光法检测抗核抗体,蛋白质印迹法检测抗ENA抗体谱.结果 原发性肺癌患者组血清自身抗体阳性检出率高于对照组（P＜0.05）;术前肺癌患者和术后肺癌患者自身抗体阳性检出率无统计学差异（P＞0.05）;肺癌患者和正常对照组在自身抗体荧光模式、抗ENA抗体谱上均存在差异.结论 肺癌患者血清中存在自身抗体,不论是手术切除前还是术后化疗阶段均可检出;其自身抗体谱与正常人生理性自身抗体谱有一定差异,进一步研究其靶抗原本质及生物学意义,有助于研究肺癌患者自身免疫现象,并可能用于肺癌的辅助诊断.
Objective To study the positive rate of auto - antibodies and locations of their target antigens in patients with lung carcinoma and correlation between auto - antibodies and lung carcinoma. Methods We collected 36 serum samples for the pathological diagnosis of primary lung cancer patients, including 16 cases without surgery and chemotherapy treatment yet, 20 cases in the surgical resection and chemotherapy stage. We also collected 47 examples for comparison. Anti - nuclear antibody was detected with the indirect immunofluorescence assay and anti - ENA antibody was detected with Western blotting. Results Positive rate of Primary lung cancer patients with serum autoantibody was higher than the healthy control group （ P 〈 0.05 ） while autoantibody positive rate in preoperative and postoperative patients with lung cancer had no significant difference （ P 〉 0.05 ）. A significant difference was found in the fluorescence pattern and anti - ENA antibody spectrum between the two groups. Conclusion Autoantibodies present in serum of patients with lung carcinoma, and before or after surgical excision could be detected both. The spectra of auto - antibodies in lung carcinoma patients are different from those in normal controls. Further efforts should be made to study the target antigens and their biological role and clinical significance.
出处
《医学新知》
CAS
2010年第6期579-581,共3页
New Medicine
关键词
肺肿瘤
自身抗体
间接免疫荧光法
蛋白质印迹法
Lung carcinoma
Autoantibody
Indirect immunofluorescence assay
Western blotting
