摘要
利用新型纳米材料羟基磷灰石固定化葡萄糖氧化酶。确定了固定化条件,考察了温度、pH、金属离子、储存时间对游离酶和固定化酶活力的影响,并测定了酶活回收率。确定的固定化最优条件为:给酶量为35ml/g,温度为30℃,pH6.5,固定时间15h。固定化酶的最适反应温度40℃,最适pH值为6.5,较游离酶分别提高了4℃和0.9。固定化酶的平均酶活回收率为61.85%。此外,固定化后的酶储存稳定性有所提高,可重复使用七次,酶剩余活力仍在62.1%。
Glucose oxidase is immobilized using a new nano-materials Hydroxyapatite.Determine the immobilization conditions,inspect the activity of the free enzyme and immobilized enzyme on temperature, pH, metal ions and storage time, and determined the activity recovery. Determine the optimum conditions for immobilization： the amount of enzyme was 35ml / g, temperature 30 ℃, pH6.5, fixed time 15h. The optimum of immobilized enzyme at 40 ℃, the optimum pH value of 6.5, compared with free enzyme were increased by 4℃ and 0.9.The average activity of the immobilized enzyme recovery was 61.32%. In addition, the immobilized enzyme increased storage stability, can be reused seven times, the remaining enzyme activity was still 62.1%
出处
《山东食品发酵》
2010年第4期14-17,共4页
Shandong Food Ferment
