摘要
为进一步研究单核细胞增多性李氏杆菌的致病机制,培养浓度为9×109 cfu/mL单核细胞增多性李氏杆菌,采用Karber法测得该菌对小鼠的LD50为2.85×108 cfu/mL。由此建立小鼠模型,然后人工腹腔感染小鼠30只,分别在感染后2h、4h、6h、8h、10h、12h、24h、48h和72h定期剖杀感染小鼠,取其脏器,采用PCR方法、分离培养及病理组织学方法,对感染模型小鼠体内的单核细胞增多性李氏杆菌动态分布及各器官的病理组织学变化进行初步研究。结果显示:感染2h,脾、肝、心血组织中均可检测到单核增多性李氏杆菌DNA,同时从上述脏器也可分离到单核增多性李氏杆菌;感染6h,大脑中可检测到单核增多性李氏杆菌DNA,并在感染后72h可分离到该细菌;感染4～6h,脾、心血、肝脑即开始出现病理变化和组织学变化。
In order to find out pathogenic mechanism for Listeria monocytogenes,cultivating Listeria monocytogenes whose concentration is 9×1010 cfu/mL,LD50 of the bacteria is 2.85×108 cfu/mL caculated with Karber.27 mices infected through abdominal by Listeria monocytogenes are killed at 2 h,4 h,6 h,8 h,10 h,12 h,24 h,48 h,72 h.Distribution of Listeria monocytogenes and pathological changes in different argans are studied in artificial infectious mice by PCR,cultivation and pathohistoloy.Listeria monocytogenes and its DNA can be detected in spleen,liver,kidney,cardiac muscle at 2 h after artificial infectious mouse and Listeria monocytogenes DNA can be detected in encephalon at 6 h,Listeria monocytogenes can be detected in the tissues at 72 h.Significant histopathological changes are observed during 4～6 h in spleen,kidney,cardiac muscle,liver,encephalon.
出处
《石河子大学学报（自然科学版）》
CAS
2010年第6期708-712,共5页
Journal of Shihezi University(Natural Science)
基金
教育部春晖计划项目(500003120702)
