摘要
以三因素三水平的正交试验设计培养条件，接种量５％（Ｖ／ｖ），ｐＨ＝６．５，１２０ｒ／ｍｉｎ，１８℃，７２ｈ，对虫草菌进行液体培养．通过对生长的菌丝体干重的测定及分析，得到该菌种生长最适培养基配方为：葡萄糖，２０ｇ／１０００ｍｌ；ＮＨ４ＮＯ３，１ｇ／１０００ｍｌ；Ｋ２ＨＰＯ４，１ｇ／１０００ｍｌ．
The optimum culture condition of C.sinsensis was investigated by the orthogonal experiment in three factors and three levels of nutrient concentration. The basic culture conditions were the quantity of inoculation, 5%; pH6.5; 120 rpm; culture temparature ,18℃; culture duration 72 h. The optimum culture condition was selected by testing and analysing desiccant weights of the collected mycelium. It was C 6H 12 O 6(glucose), 20 g/L; NH 4NO 3, 1 g/L; K 2HPO 4, 1 g/L. The carbon conpound was the most important tropho factor for the growth of C.Sinsensis.
关键词
虫草
菌丝体
培养基
正交试验
C.sinsensis mycelium substratum factor level
