摘要
采用热水浸提法,在90℃条件下水浴回流提取粽叶多糖;以sevag法、透析、醇析、SephadexG-100柱层析对粽叶多糖纯化;以Molish试剂和2.5%茚三酮乙醇试剂、紫外光谱检测纯化效果;以红外光谱测定粽叶多糖的分子结构。结果表明:用此法提取多糖分离纯化效果较优。红外光谱显示粽叶多糖组分在3 600～3 200 cm-1、3 200～2 800 cm-1、1 400～1 200 cm-1和1 200～1 000 cm-1谱段内都出现了典型多糖物质的吸收峰,在890 cm-1的吸收峰表明多糖结构中存在β2糖苷键结构,在820 cm-1处吸收峰有α-D-半乳吡喃糖的特征吸收峰。
By using hot water reflux extraction method,the polysaccharides were extracted from indocalamus in 90℃ water bath;by using sevag law,dialysis,alcohol precipitation,SephadexG-100 column chromatography to purify polysaccharides extracted from indocalamus;by using Molish reagents,2.5% of ninhydrin ethanol reagent,and ultraviolet spectrum to verify the effect of purification;by using infrared spectroscopy to determine the molecular structure of polysaccharides from indocalamus.The results showed that using these methods to extraction separation polysaccharides from indocalamus had the best effects;the infrared spectroscopy showed that polysaccharides of indocalamus showed a typical absorption peak in 3 600~3 200 cm-1,3 200~2 800 cm-1,1 400~1 200 cm-1,and 1 200~1 000 cm-1,and the absorption peak in the 890 cm-1 indicated that there is β2 glycosidic bond structure in polysaccharides structure,which is the characteristic absorption peak of α-D-galactosyl pyranose.
出处
《湖南农业科学》
2011年第1期103-106,共4页
Hunan Agricultural Sciences
基金
湖南省教育厅科研项目(08C462)
关键词
粽叶
多糖
分离
提纯
光谱分析
indocalamus leaf
polysaccharide
extraction
purification
spectral analysis
