摘要
利用维生素C的还原性将Fe^3＋还原为Fe^2＋，在pH值=4条件下，用铬天青S作Fe^3＋的显色剂，然后测量吸光度，从而建立了一种测定药物中维生素C的新方法。Fe^3＋-铬天青S的最大吸收在576nm，维生素C浓度在0-2mg／L范围内服从朗伯-比尔定律。其回归方程为A=0．0037C-0．0083，相关系数R为0．9989。实验证明，此法用于药物中维生素C的测定，效果良好。
Vitamin C was determined by CAS spectrophotometric method using iron （Ⅲ） as the oxidizer of vitamin C. CAS was used as the colorimetric reagent for iron （Ⅲ） in the pH value = 4.0. The violet complex exhibited the absorption peak at 576nm where the Lambert - Beer law was obeyed in the Vitamin C concentration range of 0 - 2mg,/L. The linear regression equation was A = 0. 0037C -0. 0083 and its relative coefficient was 0. 9989. The proposed new method was applied to the determination of Vitamin C in medicine with satisfactory results.
出处
《山东化工》
CAS
2011年第1期26-28,共3页
Shandong Chemical Industry
关键词
维生素C
铬天青S
褪色光度法
Vitamin C
CAS
fading spectrophotometric method
