摘要
利用Fenton试剂作为氧化剂,建立测定叶酸含量的间接荧光法。在pH5.2HAc-NaAc缓冲溶液中,80℃水浴中加热8min,Fenton试剂产生的羟自由基可将叶酸氧化为蝶吟-6-羧酸,氧化产物的荧光值较叶酸的荧光值大大增强,其荧光强度与叶酸质量分数在0.02～1.0mg/L范围内呈线性关系,检出限为2.69μg/L,平均加标回收率为91.0%～96.1%。将此方法应用到梨、苹果、卷心菜中叶酸含量的测定,结果满意。
The development of an indirect fluorimetric method for the determination of folic acid using Fenton reagent as oxidant is presented in this paper on the basis of the fact that after heating in a water bath at 80 ℃ for 8 min,hydroxyl radicals generated from Fenton reagent oxidize folic acid into pterin-6-carboxylic acid,whose fluorescence intensity is much higher than that of folic acid. The method displayed a linear relationship between fluorescence intensity and folic acid concentration over a range from 0.02 to 1.0 mg/L. The limit of detection was 2.69μg/L. The average recoveries for folic acid in pear,apple and cabbage separately spiked at three levels were between 91.0% and 96.1%. This method has been applied to determine the three fruits or vegetables,with satisfactory results.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第4期212-215,共4页
Food Science
基金
廊坊市科技局科学技术研究与发展计划项目(2009012017)
