摘要
将传统亚锡法加以改进，采用亚锡－葡庚糖酸钠还原剂进行肝癌片段抗体ＨＡｂ１８Ｆ（ａｂ′）２的９９Ｔｃｍ标记，整个标记过程可在１．５ｈ内完成。Ｗｈａｔｍａｎ３ＭＭ纸层析法测得最佳标记率为８４．２％。标记物室温放置２４ｈ的解离率＜５％。标记物在抗体和ＥＤＴＡ摩尔比为１∶１００００的ＥＤＴＡ介质中解离率低，而在抗体和Ｌ－半胱氨酸摩尔比为１∶６２５的Ｌ－半胱氨酸介质中解离率约为１０％。活细胞放射免疫结合法测得标记物的免疫活性分数为０．８４。荷人肝癌移植瘤裸鼠尾静脉注射标记抗体后，２４ｈ肿瘤放射性浓聚最强。
An improved pretinning method of direct labeling of anti human hepatoma monoclonal antibody fragment with 99 Tc m by using stannous glucoheptonate as reducing agent is investigated. The labeling p rocedure is finished within 1.5 h. The number of thiols in the reduced F(ab′) 2 is determined by Ellman ,s reagent, and labeling efficiency of 84.2% is obta in ed. Challenge studies indicated that 99m Tc labeled HAb18 F(ab′) 2 in EDTA is more stable than in L cysteine. The imaging result showes a s ignificant tumo r uptake at 24 h post injection of 99 Tc m HAb18F(ab′) 2. It s uggests that 99 Tc m HAb18 F(ab′) 2 conjugate prepared by this direct method appear an effect ive ability to detect hepatoma in nude mice model.
出处
《同位素》
CAS
1999年第2期90-94,共5页
Journal of Isotopes
基金
国家自然科学基金
