摘要
目的观察具有合成纤溶酶原激活物抑制物１（ｐｌａｓｍｉｎｏｇｅｎａｃｔｉｖａｔｏｒｉｎｈｉｂｉｔｏｒ１，ＰＡＩ１）蛋白酶的虹膜、晶体上皮细胞在晶体摘除联合人工晶体植入术后其合成的ＰＡＩ１量的变化，以阐述ＰＡＩ１对白内障囊外摘除联合人工晶体植入术后纤维蛋白反应和后囊膜混浊形成的作用。方法８只新西兰白兔，均１只眼经晶体囊外摘除术后在晶体囊袋内植入人工晶体，另１只眼未手术作为正常对照；每２只兔眼（２只手术眼和２只未手术眼）分别在术后第１、７、１５、３０天时，取出整个虹膜和晶体囊膜组织，抽取ＲＮＡ；应用逆反转录聚合酶链反应技术观测各标本中ＰＡＩ１ｍＲＮＡ表达量的变化，数据进行统计学分析处理。结果在术后第１天，虹膜和晶体上皮细胞的ＰＡＩ１ｍＲＮＡ表达量即出现明显的升高；在虹膜组织中，ＰＡＩ１ｍＲＮＡ这种高水平表达可持续到术后１５天（Ｐ＜０．００１），但在术后３０天时，其表达量又降至正常（Ｐ＝０．８７）；而晶体上皮细胞ＰＡＩ１ｍＲＮＡ的表达量，在术后１、７、１５、３０天时术眼显著高于正常对侧眼（Ｐ＜０．００１）。结论ＰＡＩ１可能是引起白内障囊外摘除联合人工晶体植入术后纤维蛋白反应的主要原因，同时它对?
Objective To investigate the change of plasminogen activator inhibitor1 (PAI1) synthesized by the iris and the lens epithelial cells (LECs) after extracapsular cataract extraction (ECCE) with intraocular lens (IOL) implantation and the role of PAI1 in the occurrence of fibrin reaction and the formation of posterior capsular opacification. Methods Eight adult rabbits were studied. In each rabbit, one eye underwent ECCE with IOL implantation and the other eye was normal without any surgical intervention. The iris tissue and the lens capsule of each two normal eyes and each two surgical eyes were excised on the postoperative 1, 7, 15 and 30 days respectively. The amount of PAI1 mRNA expresson in the specimens was detected by using reverse transcriptionpolymerase chain reaction (RTPCR). The data were analyzed statistically. Results The amount of PAI1 mRNA expresson in the iris tissue and the LECs of the surgical eyes displayed a significant increase on the first day after operation. The high expression of PAI1 mRNA by the iris might last 15 days (P<0.001), but it declined to the normal range on the postoperative 30 days (P=0.87). The high level of PAI1 mRNA expresson by the LECs in the surgical eyes had significant difference when compared with that of the normal eyes at 1, 7, 15 and 30 days postoperatively (P<0.001). Conclusion It is indicated that PAI1 enzyme is a major cause for fibrin reaction after ECCE with IOL implantation, and may play critical roles in the development of posterior capsular opacification.
出处
《中华眼科杂志》
CSCD
北大核心
1999年第3期223-226,共4页
Chinese Journal of Ophthalmology
