摘要
以芋头块茎组织为供试材料,用来自石榴、甘薯和芋头的甘薯长喙壳(Ceratocystis fimbriata Ellis et Halsted)菌株分别接种芋头块茎,同期观察3种不同致病性的菌株在互作中芋头过氧化物酶(POD)、超氧歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量的变化。结果表明:接种3个菌株5 h后,芋头块茎组织开始呈微红色,对照块茎组织无颜色变化;芋头菌株和石榴菌株均可在芋头块茎组织表面生长,并最终致其腐烂,表现出亲和反应;接种甘薯菌株后芋头块茎组织表面无菌丝生长,表现出非亲和反应。同期酶活性及膜脂过氧化结果显示:在接种25～30 h后,芋头块茎组织中POD、SOD和CAT活性达到高峰,且非亲和接种(甘薯菌株)块茎组织高于亲和接种(芋头菌株、石榴菌株)块茎组织;在接种10 h后,芋头块茎组织中MDA含量明显升高,其表面的颜色呈浅褐红色,在接种20 h后含量达到最高值,随后始终维持较高水平,且非亲和接种块茎组织高于亲和接种块茎组织。
A taro strain （compatible or pathogenic）, a pomegranate（Punica granatum） strain （compatible or pathogenic） and a sweet potato strain （incompatible or non-pathogenic） of Ceratocystis fimbriata Ellis et Halsted, were inoculated into taro （Colocasia esculenta Schott） tubers. Subsequently, the changes of the activities of peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD） and the content of malondialdehyde （MDA） in inoculated taro disks were observed. The results showed that the surfaces of the taro tuber inoculated by all the strains turned reddish under the same conditions after 10 h of inoculation. The taro strain and the pomegranate strain were pathogenic to and compatible with taro, while the sweet potato strain was non-pathogenic to and incompatible with taro. POD, SOD, CAT increased to a higher level in incompatible （sweet potato） strain than those in compatible （pomegranate and taro） strains after 25-30 h inoculation with those pathogens respectively. The surfaces of the taro tuber inoculated by all the strains turned mahogany after 10 h inoculation, whose MDA increased up to a peak at 20 h.
出处
《华中农业大学学报》
CAS
CSCD
北大核心
2011年第1期61-65,共5页
Journal of Huazhong Agricultural University
基金
云南省科技攻关计划(2003NG08)
教育部博士点基金项目(20095302110003)
关键词
甘薯长喙壳
芋头
抗氧化酶
Ceratocystisfimbriata Ellis et Halsted
taro
antioxidant enzyme
