摘要
以2月龄袋装麻楝实生苗为材料,用0、100、500和1 000 mg.L-1稀土喷液施麻楝幼苗,后置于3℃人工气候箱低温处理1～7 d,考察其膜稳定性、渗透调节物质和叶绿素含量等抗寒性生理生化指标的变化,探讨稀土对麻楝幼苗抗寒性的影响。结果表明:0和100 mg.L-1稀土低温各天数后的相对电导率显著大于0 d,500和1 000 mg.L-1处理的显著小于0 d。各稀土浓度处理的幼苗脯氨酸含量都显著大于0 d,可溶性蛋白质含量多小于0 d,叶绿素含量多大于0 d。0和100 mg.L-1稀土处理的幼苗丙二醛含量大于0 d或与0 d无显著差异。500和1 000 mg.L-1稀土处理的幼苗各低温天数的丙二醛含量与0 d相近或小于0 d。0 mg.L-1稀土处理后,幼苗各天数的SOD活性显著小于0 d,100 mg.L-1处理的波动,500和1 000 mg.L-1处理的各天数的SOD活性与0 d的差异不显著。可见,在低温胁迫下,500和1 000 mg.L-1的稀土处理能通过提高渗透调节物质含量来稳定麻楝幼苗细胞膜稳定性,从而增强麻楝幼苗的抗寒性。
Chukrusia tabularis seedlings were laid on low temperature of 3℃ in artificial climatic box from one to seven days after they were sprayed with rare earth of 100,500 and 1 000 mg · L- 1, respectively, and then changes of their physiological and biochemical indices, such as membrane stability, osmoregulation substance and chloro phyll content were studied. The results show that the relative electrical conductivity of the seedlings sprayed with rare earth of 0 and 100 mg· L- 1 was significantly greater than that of 0 day , whereas that sprayed with rare earth of 500 and 1 000 mg· L- 1 was significantly smaller than that of 0 day under all low temperature treatments. The content of free praline sprayed with rare earth of all concentrations was significantly greater than that of 0 day and their soluble protein concentrations generally smaller than that of 0 day, whereas their chlorophyll content generally greater than that of 0 day. The MDA content of seedlings sprayed with rare earth of 0 and 100 mg · L -1 was grea ter than that of 0 day or not significant difference from the latter, whereas that sprayed with rare earth of 500 and 1 000 mg· L-1 was smaller than that of 0 day or similar to the latter. The SOD activity of the seedlings sprayed with rare earth of 0 and 100 mg · L- 1 was significantly smaller than that of 0-day treatment, and that sprayed with rare earth of 100 mg · L-1 fluctuated, whereas that sprayed with rare earth of 500 and 1 000 mg · L -1 was not sig nificant difference from that of 0-day treatment. In this study, the seedlings sprayed with rare earth of 500 and 1 000 mg · L -1 increased membrane stability by enhancing osmoregulation substance content, thereby improved their cold resistance ability.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2011年第2期34-40,共7页
Journal of Central South University of Forestry & Technology
基金
广东省林业局资助项目"筛选林分改造优良树种"(4400-F09054)和"森林生态科技研究与推广"
关键词
稀土
低温胁迫
麻楝
生理指标
rare earth
Chukrasia tabularis
cold resistance
physiological index
