摘要
为了研究胞浆３磷酸甘油脱氢酶（ｃｔＧＰＤ， ＥＣ １．１．１．８）在产甘油假丝酵母（ ＣａｎｄｉｄａｇｌｙｃｅｒｏｌｇｅｎｅｓｉｓＺｈｕｇｅ ）甘油合成机制中的作用，考察了发酵实验过程中ｃｔＧＰＤ的活力水平以及高渗环境对该酶酶活水平和甘油形成的影响．结果表明，该菌种中，ｃｔＧＰＤ 的表达在相对低渗的发酵过程中处于一个较高的水平，并在３６ ｈ 和６０ ｈ 处分别出现两次峰值；高渗环境可使ｃｔＧＰＤ酶活增加，但幅度不大．发酵试验表明，随着高渗溶质浓度增大，可在一定程度上恢复细胞产甘油能力，但总水平下降．
Candida glycerolgenesis Zhuge is characterized by its osmotolerance and over production of glycerol. A 120 hours' fermentation was processed and ctGPD was found to maintain at a quite high and steady level. Two peaks of activity appeared at the moments of 36 hours and 60 hours. The ctGPD could be induced by NaCl,KCl and mannitol but the specific activity didn't increase remarkably. The fermentation results indicated that the yield of glycerol decreased and the capacity of producing glycerol per cell increased with the solutes concentration but is still less than that of the contrast.
基金
国家九五科技基金
关键词
甘油
产甘油假丝酵母
磷酸甘油脱氢酶
发酵法
Glycerol
Candida glycerolgenesis
Cytoplasmic glycerol 3 phosphate dehydrogenase
