摘要
在摇瓶条件下， 以淀粉为碳源，蛋白胨及酵母膏为氮源，对Ｓｔｒｅｐｔｏｖｅｒｔｉｃｉｌｌｉｕｍｍ ｏｂａｒａｅｎｓｅ生产谷氨酰胺转胺酶（ＭＴＧ）的摇瓶发酵条件进行了探索．研究结果表明，发酵法生产谷氨酰转胺酶的适宜初始ｐＨ 值、淀粉质量浓度、接种量分别为６．１，２ ｇ／ｄＬ，１０％ ；ＭＴＧ酶活最高可达１．８３ ｕｍ ｏｌ／（ｍ ｉｎ·ｍ Ｌ）．初始加入硫酸铵对菌体生长影响不大，但对ＭＴＧ 的合成却产生抑制作用；初始加入纯氨基酸及胱氨酸母液不利于ＭＴＧ 酶活的提高．
The conditions of shaking-flask culture for the MTG production were investigated with Streptoverticillium morbaraense with starch as carbon source,peptone and yeast extract as nitrgon source.The results indicated that the proper pH,the initial starch concentration and the size of inoculum were 6.05,2%,and 10% respectively.The effect of the initial concentration of ammomium sulfate on cell dry weight was not obvious,but the addition of ammonium sulfate inhibited the production of MTG.The initial addition of free amino acids and liquids of cystamine had a bad effect on the production of MTG.The highest enayme activity reached 1.83 μmol/(min·mL).
关键词
谷氨酰胺转胺酶
摇瓶发酵
发酵
生产
MTG
Streptoverticillium morbaraense
transglutaminase
shake-flask culture.
