摘要
【目的】分析不同浓度CaCl2及钙作用抑制剂EGTA(钙螯合剂)与TFP(钙调素抑制剂)对亚高温下番茄叶片光合作用及PSⅡ与PSⅠ荧光参数的影响,探讨钙对亚高温下番茄叶片光合作用的调控机制。【方法】以‘辽园多丽’番茄幼苗为试材,通过0、10、30 mg.L-1的CaCl2预处理,筛选出防止亚高温逆境障碍的适宜CaCl2浓度,进而通过喷施CaCl2及其作用抑制剂(EGTA与TFP)预处理,探讨昼间35℃亚高温下钙对番茄叶片光合作用的调控作用。【结果】不同浓度CaCl2处理均对亚高温下番茄叶片的净光合速率有提高作用,其中10 mg.L-1CaCl2处理效果最显著;CaCl2处理显著提高了亚高温逆境下叶片PSⅡ的最大光化学效率(FPSII),显著提高了PSⅡ与PSⅠ的量子产量[Y(Ⅱ)与Y(I)],提高了由于供体侧限制引起的PS I处非光化学能量耗散的量子产量[Y(ND)],降低了由于受体侧限制引起的PS I处非光化学能量耗散的量子产量[Y(NA)],而EGTA与TFP则起到一定负调控作用。【结论】喷施10 mg.L-1CaCl2能够有效提高亚高温下番茄叶片光合作用,钙对于维持气孔的正常开闭及保护PSⅡ与PSⅠ活性均具有重要调控作用。
【Objective】In order to reveal the regulation function of calcium on photosynthesis of tomato leaves under sub-high temperature stress,the effects of CaCl2 on photosynthesis and fluorescence of PS Ⅱand PS Ⅰ under sub-high temperature stress were studied.【Method】In order to select a appropriate concentration,the tomatoes grown in greenhouse were pretreated with 0,10 and 30 mg·L-1CaCl2 during the period of sub-high temperature,and then photosynthesis,chlorophyll fluorescence of PS Ⅱand PS Ⅰ were analyzed for the tomatoes pretreated with CaCl2,with the appropriate concentration,Ca2＋ chelator EGTA and CaM antagonist TFP.【Result】The net photosynthetic rate（Pn） increased at three concentrations of CaCl2 treatments under sub-high temperature stress,especially in the treatment of 10 mg·L-1CaCl2.Under sub-high temperature stress,the maximal photochemical efficiency（FPSII） of PS Ⅱ,photochemical quantum yield of PS Ⅱ and PS Ⅰ[Y（Ⅱ） and Y（Ⅰ）],quantum yield of non-photochemical energy dissipation in PS I due to donor side limitation in the tomato leaves pretreated with CaCl2 were much higher than that of control,while quantum yield of non-photochemical energy dissipation in PS I due to acceptor side limitation was much less than that of the control.EGTA and TFP treatments had negative effect.【Conclusion】The treatment of 10 mg·L-1 CaCl2 could improve photosynthesis of tomato leaves under sub-high temperature stress.And calcium played an important role in regulating stomatal movements and protecting PS Ⅰ PS Ⅱ not to be injured.
出处
《中国农业科学》
CAS
CSCD
北大核心
2011年第3期531-537,共7页
Scientia Agricultura Sinica
基金
国家农业科技成果转化资金项目(2009GB2B000065)
公益性行业(农业)科研专项(nyhyzx07-007)
辽宁省教育厅创新团队项目(2008T174)
国家现代农业产业体系建设专项(Nycytx-35-gw23)
关键词
番茄
光合作用
叶绿素荧光
PSⅡ
PSⅠ
钙
tomato
photosynthesis
chlorophyll fluorescence
PS Ⅱ
PS Ⅰ
calcium
