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耐力运动和高脂膳食对大鼠肌纤维钙调神经磷酸酶蛋白含量和活性的影响

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摘要 目的分析耐力运动和高脂膳食与钙调神经磷酸酶(CaN)信号通路的关系。方法雄性SD大鼠(150~180 g)40只随机等分成4组:对照组、运动组、高脂组、运动高脂组。第6周末取比目鱼肌(SOL)和趾长伸肌(EDL)进行分析。用比色法测胞浆和胞核CaN活性,并用Western blotting法测胞浆和胞核CaN蛋白含量。结果耐力运动和高脂膳食对SOL静息CaN蛋白和活性无影响。高脂膳食降低EDL胞浆CaN活性,而耐力运动升高EDL胞浆CaN活性,但皆不影响CaN蛋白含量。结论耐力运动和高脂膳食诱导的趾长伸肌纤维大小改变与CaN活性相关。
出处 《广东医学》 CAS CSCD 北大核心 2011年第2期144-145,共2页 Guangdong Medical Journal
基金 广东省自然科学基金资助项目(编号:8151007601000001)
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