摘要
目的探讨孕期脂多糖刺激诱导子代大鼠（简称子鼠）高血压的机制研究。方法将SD孕鼠6只随机分为两组。对照组3只：腹腔注射无菌生理盐水0.5 mL;脂多糖组3只：腹腔注射脂多糖,剂量为0.79 mg/kg。分别在孕期第8、10、12天08∶00~09∶00进行腹腔注射。子鼠出生后,观察7~25周龄子鼠血压的变化和肾组织肾素mRNA表达、血管紧张素Ⅱ1型受体（AT1-R）的蛋白表达以及肾组织血管紧张素Ⅱ（AngⅡ）阳性细胞表达。结果与对照组相比,脂多糖组子鼠血压逐渐升高,至24周龄时达到高血压水平。血浆肾素活性和AngⅡ浓度无变化。7、16、25周龄子鼠肾内肾素mRNA表达和AngⅡ阳性细胞数与对照组相比明显增高,并与血压呈正相关;AT1-R的蛋白表达与对照组相比降低,与肾素mRNA表达呈负相关。结论孕期暴露于脂多糖引起子代成年大鼠肾组织肾素-血管紧张素系统活性增高,这可能是血压升高的一个机制。
Objective To explore the mechanism of hypertension caused by maternal exposure to lipopolysaccharide in adult offspring rats.Methods Nulliparous,pregnant,time-matched SD rats were randomly divided into two groups（n=3）：control group and LPS group.On the pregnant day 8,10 and 12,rats in control group and LPS group were administered intraperitoneally with saline 0.5 mL or LPS 0.79 mg/kg,respectively.The systolic blood pressure was measured in offspring once three weeks from 7 weeks of age to 25 weeks of age.The plasma renin activity and angiotensin II concentration were measured in adult offspring rats at 7,16 and 25 weeks of age,intrarenal renin was assessed by real-time PCR,AT1-R protein expression was assessed by Western blot and angiotensin Ⅱ-positive cells were determined with immunohistochemical staining.Results The blood pressure in offspring of LPS-treated rats increased constantly and reached hypertensive level at 24 weeks of age.The intrarenal renin mRNA expression and the number of angiotensin Ⅱ-positive cells increased at 7,16 and 25 weeks of age,while the AT1-R protein expression decreased.The plasma renin activity and angiotensin Ⅱ were unchanged.Conclusion Maternal exposure to lipopolysaccharide activates intrarenal renin-angiotensin system in adult offspring rats.
出处
《重庆医学》
CAS
CSCD
北大核心
2011年第5期445-447,F0003,共4页
Chongqing medicine
关键词
炎症
脂多糖类
肾素-血管紧张素系统
高血压
inflammation
lipopolysaccharides
offspring
renin-angiotensin system
hypertension
