摘要
伪狂犬病活疫苗作为一种安全有效、全国广泛推广使用的疫苗,其效力检验十分必要,而伪狂犬强毒毒价测定则是效力检验的一个必要前提。本实验选用BHK-21细胞及家兔和小鼠实验动物,来检测PRV的TCID50和LD50,旨在找出基于家兔和小鼠两种实验动物PRVTCID50和LD50之间的关系,以简化该苗的效力检验过程。结果表明:用兔子做效力检验时,可以用TCID50代替测LD50。
Pseudorabies vaccine as a safe and effective vaccine is widely used in the country,and it is extremely necessary to examine its effectiveness.The PRV virulence test is the necessary prerequisite for the efficacy test.BHK-21 cell and rabbits and mice were used in the experiment to detect PRV TCID50 and LD50,to identify the relationship of TCID50 and LD50 of PRV based on the two kinds of experiments on rabbits and mice.The results showed that in the efficacy test with rabbits,TCID50 could substitute LD50.
出处
《中国动物检疫》
CAS
2011年第1期52-54,共3页
China Animal Health Inspection
基金
滨州市应用技术研究与开发专项(200706)
院青年科技创新基金项目(2007-02)
