摘要
以百合品种元帅(Acapulco)、卡萨布兰卡(Casablanca)的愈伤组织为试验材料,附加不同浓度的枯萎病菌毒素粗提液,用于抗尖孢镰刀菌无性系的离体筛选。结果表明,以体积分数75%的毒素培养基上培养10 d作为百合抗尖孢镰刀菌无性系筛选的选择压比较适宜,抗镰刀菌无性系的再生苗生长正常,叶片中过氧化物酶(POD)、多酚氧化酶(PPO)、超氧化物歧化酶(SOD)活性均比未经筛选的对照株高,经人工接种枯萎病菌鉴定,2个百合品种抗尖孢镰刀菌无性系的再生植株均表现中抗枯萎病。
In order to obtain anti-Fusarium clones of lily,callus of Acapulco and Casablanca were employed as material for the seclection of anti-Fusarium clones by adding various concentrations of toxin in MS medium.The result showed that medium containing 75% of the toxin and 10 d culture can be used as selection pressure for screening lily anti-Fusarium clones and its regeneration plants of disease-resistance had healthy growth.It was found that activity of three enzymes,POD,PPO and SOD,in screened plants was higher than that of control and the screened plants were healthy.Lily anti-Fusarium clones showed a middle level of resistance to lily blight disease by identification of disease-resistance levels.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2011年第1期34-38,共5页
Journal of Hunan Agricultural University(Natural Sciences)
基金
云南省科技计划项目(2006NG14)
国家科技支撑计划项目(2006BAD01A1803
2007BAD45B01)
云南省社会发展科技计划项目(2009ZC140M)
关键词
百合
尖孢镰刀菌
无性系
枯萎病
lily
Fusarium oxysporum f.sp.lilii
clones
wilt
