摘要
从丹阳市某牛蛙养殖场采集发病牛蛙,在其肠、肝、脾、肾和腹腔积液中分离出2株菌株,根据菌体形态特征、生理生化特性,应用API20E细菌生化快速鉴定试剂条进行鉴定,结果显示:其中1株为嗜水气单胞菌(Aeromonas hvdrophila),另1株为类志贺邻单胞菌(Plesiomonas shigelloides)。经人工感染健康牛蛙,其中嗜水气单胞菌表现出较强的致病力,发病症状与自然发病牛蛙相同,证实这株菌为牛蛙发病的病原菌。药敏试验结果表明:该致病菌对丁胺卡那、头孢呋新和庆大霉素有较高的敏感性。
The bacterial pathogens were isolated from their bowel,liver, spleen、kidney and celiac effusion of the diseased bullfrogs, which were cultured in a breed farm in Danyang county,Jiangsu province. They were. identificated by API20E based on their shapes and physiological charateristics. The results showed these bacterial pathogens were Aeromonas hvdrophila and Plesiomonas shigelloides. Through manual--infected to healthy bullfrogs, Aeromonas hvdrophila showed strong ability to induce disease and the symptom was same to the wild sick bullfrogs. This results approved that Aeromonas hvdrophila was the key pathogenic bacterium. The drug sensitivity test showed that Aeromonas hv- drophila was sensitive to AKN.CFX and GEN.
出处
《河北渔业》
2011年第2期41-43,56,共4页
基金
江苏省农业科技自主创新项目(项目编号:cx(10)218)
关键词
牛蛙
病原菌
分离鉴定
嗜水气单胞菌
药敏实验
Bullfrog
Pathogenic bacterium
Isolation and identification
Aeromonas hvdrophila
Drug sensitivity test
