摘要
相对湿度是生物学实验中一个十分重要而又较难准确控制的环境因素．本项研究设计出一种改进的简易控湿装置，可利用普通温光型培养箱同时进行不同湿度水平的生物学实验．该装置由微型气泵、３个试剂瓶串连而成的三联体和一个用有机玻璃制成的生长箱组成，经在２０℃下对饱和酒石酸钠溶液调节的湿度（理论值９２％）连续测定和校正，控湿效果良好，误差仅±１％左右．同时对不同浓度硫酸溶液控制生长箱内不同水平的湿度进行了测定和检验，亦效果良好，且对环境温度的适应性强，在５～３５℃范围内同一硫酸浓度控制相同的稳定湿度．最后。
Relative humidity is an environment factor that significantly
affects biological experiments, but is difficult to precisely control. A modified device for easy
control of the humidity in regular thermal and light growth chamber was described in this paper.
The device included three parts: 1) air pump; 2) three pipe collected containers of salt solution
for producing expected humidity; and 3) a perspex chamber, in which, experimental materials
can be placed. The modified device worked well for humidity control with an error of only 1%,
while a digital data logger was used to monitor the relative humidity controlled by saturated
solution of sodium tartrate every three minutes for 6 h at 20℃, which was supposed to yield a
92% r.h. at that temperature. Varying concentrations of sulfate acid were tested as humidity
control agents at 5～35℃, with each yielding a stable humidity in the perspex chamber
regardless of temperature. The stability of the humidity control system was also discussed
when the solutions of sulfate acid were used as humidity control agents.
出处
《应用生态学报》
CAS
CSCD
1999年第3期357-361,共5页
Chinese Journal of Applied Ecology
基金
国家杰出青年科学基金
国家自然科学基金
关键词
湿度控制
改良装置及工作原理
生物学实验
Humidity
control, Modified device and methodology, Biological experiments.
