摘要
取北五味子带腋芽的嫩茎作外植体，培养于附加不同种类和浓度激素的ＭＳ培养基上。在附国加６－ ＢＡ２．０ ＋ ＺＴ０．１ｍｇ／Ｌ时，诱导芽的效果最佳；在附加６ －ＢＡ２．０ ＋ ＮＡＡ０．２ ＋ ＺＴ０．１ｍｇ／Ｌ时，芽的增殖率最好；在附加ＮＡＡ０．２ｍｇ／Ｌ时，生根效果最佳。当苗高２．０～２ ．５ｃｍ ，根数达３～５ 条时，开瓶锻炼２ 天，移栽于消过毒的土壤中，１０ 天后，成活率达１００％ 。
The explants of Chinese magnoliavine tender stems with axillary buds are cultured in MS medium with addition of different concentration and kinds of hormone. The results show that sprouting of axillary bud is best on MS medium with addition of 6-BA 2.0 +ZT 0.1 mg/L;and the multiplication of adventitious bud is best on MS medium with the addition of 6-BA 2.0 +NAA 0.2 +ZT 0.1 mg/L;and the growth of roots is best on MS medium with addition of NAA 0.2 mg/L. Seedlings with growing to 2.0～2.5cm and 3～5 roots are transplanted to the pots with disinfect soil after 2 days training. 10 days later, the survival rate reaches 100%.
出处
《植物研究》
CAS
CSCD
北大核心
1999年第3期318-322,共5页
Bulletin of Botanical Research
关键词
北五味子
外植体
组织培养
Explant
Chinese magnoliavine
Tissue culture
