摘要
目的通过观察口服维生素A对大鼠哮喘模型的气道炎症细胞及肺组织中TNF-α的影响,初步判定口服维生素A对哮喘的治疗效果及作用机制。方法将实验动物随机分成两组:哮喘组、维生素A口服组。以卵清蛋白(OVA)致敏激发建立哮喘模型。维生素A口服组在实验第21 d行维生素A口服治疗一周。实验第28 d和第35 d收集大鼠BALF,进行BALF中细胞总数及嗜酸性粒细胞(EOS)计数,并用酶联免疫吸附(ELISA)法检测各组肺组织中TNF-α水平。结果与哮喘组相比,维生素A口服组炎症细胞数和嗜酸性粒细胞明显下降,但淋巴细胞及巨噬细胞比例增加,第一周肺组织中TNF-α含量无明显改变,第二周则下降明显,具有统计学意义。结论口服维生素A在治疗哮喘时有一定的疗效,它可能在治疗哮喘初期加重肺部炎症反应,但在恢复期可使肺部的炎症较早消退,病程大大缩短。
Objective To observe the effect of vitamin A on airway inflammatory cells and TNF-α of lung tissue in rats with asthma and to discuss the effect of Vitamin A in asthma cure and its mechanism.Methods To divide 32 female SD rats into 2 groups as following：Asthma group and Vitamin A group.The Seventh day and 14th day each executed 1/2 amount of animals after Vitamin A intervention.The level of TNF-α in lung tissue was measured by enzyme-linked immunosorbent assay.The cell numbers in BALF were counted as total cells,eosinophils、Lymphocytes and macrophages.Results Compared with Asthma group,Vitamin A group can decrease the amount of the total cells and EOS,But increased the lymphocyte and macrophage,in the first week,the amount of TNF-α in lung tissue did not change significantly,in The second week the amount of TNF-α obviously decreased.Conclusion Vitamin A in the treatment of asthma have certain curative effect,Early in the treatment of asthma,It may be aggravated lung inflammation,but It can make the lung inflammation to subsidise earlier in last phase and shorten the duration Greatly.
出处
《临床肺科杂志》
2011年第3期376-378,共3页
Journal of Clinical Pulmonary Medicine
