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高效液相色谱法同时测定四物合剂中4种活性成分的含量 被引量:1

HPLC simultaneous determination of paeoniflorin,adenosine,tetramethylpyrazine and ferulic acid in Siwu mixture
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摘要 目的:建立同时测定四物合剂中芍药苷、腺苷、川芎嗪和阿魏酸4种有效成分含量的高效液相色谱方法。方法:采用Symmetry C18色谱柱(150mm×3.9mm,5.0μm);流动相为0.05%甲酸水溶液(A)-乙腈(B),线性梯度洗脱(0~5min,10%B;5~10min,10%B→20%B;10~45min,20%B→+45%B),流速1.0mL·min^-1;柱温35℃;检测波长分别为230nm(芍药苷),260nm(腺菩),282nm(川芎嗪),323nm(阿魏酸)。结果:芍药苷、腺苷、川芎嗪和阿魏酸的质量浓度分别在9.50~190,5.70~114,3.02-603,8.00~160mg·L^-1范围内呈良好线性关系(R^2≥0.992);日内、日间精密度良好,RSD均小于1.95%(n=6);各组分低、中、高浓度的平均加样回收率均在98.5%~101.2%之间,RSD均小于1.85%(n=6);四物合剂中芍药苷、腺苷、川芎嗪和阿魏酸的质量浓度分别为(1758.0±2.1),(187.4±1.4),(78.7±0.5),(90.9±1.0)mg·L^-1。结论:本方法简便可靠,重复性好,可全面反映四物合剂中各味药的质量,适用于四物合剂质量控制。 OBJECTVE To develop an HPLC method for simultaneous determination of paeoniflorin, adenosine, tetramethylpyrazine, and ferulic acid in Siwu mixture. METHODS A Symmetry C18 column (150mm×3.9mm,5.0μm) was used. The mobile phase consisted of 0. 05% aqueous formic acid (A) and acetonitrile (B) with linear gradient elution (0 - 5 min, 10% B; 5 - 10 min, 10% - 20% B; 10 - 45 min, 20% - 45% B), The flow rate was 1.0 mL.min ^-1. DAD detection was used and the detection wavelength was set at 230 nm for paeoniflorin, 260 nm for adenosine, 282 nm for tetramethylpyrazine and 323 mn for ferulaic acid, respectively. The temperature of column was 35 ℃. RESULTS Paeoniflorin, adenosine, tetramethylpyrazine, and ferulic acid showed good linearity (R2≥0. 992) in the range of 9. 50 - 190,5. 70 - 114,3. 02 - 603 and 8. 00 - 160μg-mL ^-1 , respectively. The average recovery rates of the four investigated compounds at low, medium and high concentration levels were in the range of 98. 5%-101.2% for all with RSDs below 1.85% (n = 6). The RSDs of intra day and interday precisions were not more than 1.95% (n = 6). The contents of paeoniflorin, adenosine, tetramethylpyrazine, and ferulic acid in Siwu mixture were (1 758. 0 ± 2. 1), (1 87. 4±1. 4), (78. 7 ± 0. 5), (90. 9 ± 1.0) mg·L ^-1 , respectively. CONCLUSION The developed method is simple, accurate, reliable and can be used to control the quality of Siwu mixture.
出处 《中国医院药学杂志》 CAS CSCD 北大核心 2011年第4期286-289,共4页 Chinese Journal of Hospital Pharmacy
关键词 高效液相色谱法 四物合剂 芍药苷 腺苷 川芎嗪 阿魏酸 HPLC Siwu mixture paeoniflorin adenosine tetramethylpyrazine ferulic acid
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