摘要
目的观察PI3K/Akt在高糖诱导的肾小管上皮细胞转分化中的作用及对分化抑制因子2(Id2)表达的影响。方法大鼠肾小管上皮细胞随机分成3组:对照(Con)组,高糖(H-Glu)组和PI3K抑制剂Wortmannin(Wort)组。Con组加正常培养液,H-Glu组在培养液中加30 mmol/L葡萄糖,Wort组用Wortmannin预处理1 h后,加30 mmol/L葡萄糖。24 h后用免疫细胞化学、Western blot法和RT-PCR法检测α平滑肌肌动蛋白(α-SMA)、p-Akt和Id2的表达。结果 Con组α-SMA、p-Akt阳性表达细胞很少,绝大部分细胞出现Id2阳性表达。与Con组比较,H-Glu组出现α-SMA和p-Akt表达升高,Id2表达降低(P<0.05)。与H-Glu组比较,Wort组α-SMA和p-Akt表达下降,Id2表达升高(P<0.05)。结论 PI3K/Akt可能通过抑制Id2基因表达,参与高糖诱导的大鼠肾小管上皮细胞转分化。
Objective To observe the effects of PI3K/Akt on inhibitor of differentiation 2 in tubular epithelial myofibroblast transition induced by high glucose. Methods Normal rat kidney tubular epithelial cells were randomly divided into three groups： control group（group Con）, high glucose group （group H-Glu） and wortmannin group （group Wort）. Cells in group Wort were pretreated with wortmannin for an hour before stimulation with high glucose （30mmol/L） in group H-Glu and Wort, and cells in group Con were treated with media only. Twenty four hours later, the level of phosphorylation Akt ,Id2 and expression of a-SMA were assayed. Results Expression of p-Akt and a-SMA were very low and most of the cell had positive expression of Id2 in group Con. In group H-Glu the expression of a- SMA and p-Akt increased and Id2 decreased significantly, while in group Wort the wortmannin obviously alleviated the change induced by high glucose. Conclusions PI3K/Akt may involve in tubular epithelial- myofibroblast transition induced by high glucose through inhibiting Id2.
出处
《中国糖尿病杂志》
CAS
CSCD
北大核心
2011年第2期146-148,共3页
Chinese Journal of Diabetes
关键词
PI3K/AKT
分化抑制因子
肾小管上皮细胞
转分化
PI3K/Akt
Inhibitor of differentiation
Renal tubular epithelial cell
Transdifferentiation
