摘要
目的:采用Folin-Ciocaileu比色法测定啤酒花中的总多酚的含量。方法:以没食子酸作为对照品,以福林试剂-碳酸钠溶液体系为显色剂,检测波长765 nm。结果:标准曲线的回归方程为Y=0.046 59+109.89X(r=0.999 4),线性范围0.049～0.595 g·L-1,稳定性、精密度、重现性、回收率的RSD 1.01%～1.53%,平均回收率分别为99.6%,101.7%,98.3%。结论:该方法简便、快速、准确,是检测啤酒花中总多酚成分的可靠方法,为控制啤酒花的内在质量提供了依据。
Objective ： To estabilsh hops. Method： Folin-Ci0caileu was adopted The total polyphenol was linear in the range The average recovery was 99.7% ,101.7% producibility, and can control the quaihy of an UV method for determination of the content of total polyphenol for determining the content of total polyphenol by UV at 765 nm. Result： of 0.049-0. 595 g. L-1 with the relative standard deviation r = 0. 999 4. ,98.3%. Conclusion：The method is accurate and simple with good rethis prepararion effectively.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第5期58-60,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(30801555)
