摘要
目的 探讨新发现的肿瘤转移抑制基因KAI1/CD82与人癌细胞转移潜能的关系。方法应用Northernblot杂交及聚合酶链反应 单链构象多态性分析 (PCR SSCP)方法 ,检测 3组 8种不同转移潜能人前列腺癌细胞、肺癌细胞及黑色素瘤细胞中KAI1/CD82基因的表达及与突变的关系。结果具有转移性的前列腺癌细胞系PC3和PC3M及高转移性的人肺PG细胞 ,KAI1mRNA的表达降低 (积分吸光度分别为 0 0 3 19,0 0 2 66和 0 0 5 4 9) ,而在无转移性的人肺PAa细胞则为高表达 (积分吸光度为 0 73 13 )。 4种不同转移潜能的人黑色素瘤细胞WM3 5、WM13 41b、WM983a及WM4 5 1KAI1基因均呈中度表达 (积分吸光度分别为 0 1798、0 15 82、0 15 0 1及 0 180 0 )。PCR SSCP结果显示 ,在KAI1基因第 7外显子PC3、PG及PC3M均可见带型的泳动变位 ,PC3M较弱。结论 KAI1/CD82基因表达的降低 ,可能与前列腺癌细胞、肺癌细胞的转移有关。但与黑色素瘤细胞无关。KAI1表达的降低可能部分与基因的突变失活有关。
Objective To investigate the correlation of a newly defined tumor metastasis suppressor gene KAI1/CD82 and metastatic potential of human cancer cell lines Methods Expressions of KAI1/CD82 in eight human cancer cell lines of prostate, lung and melanoma with different metastasis potential were studied by Northern blot hybridization and the mutation were detected by PCR SSCP Results Expressions of KAI1 mRNA decreased in human prostate cancer cell lines PC3 and PC3M, and lung cancer cell line PG which were metastatic cancer cell lines (integral light density values: 0 031 9, 0 026 6 and 0 054 9, respectively) Expression of KAI1 mRNA showed high level in PAa, a human lung cancer cell line with no metastasis (integral light density value: 0 731 3) In human melanoma cell lines with different metastasis potential (WM35,WM1341b,WM983a and WM451), expression of KAI1 showed moderate expression (integral light density values are 0 179 8, 0 158 2, 0 150 1, 0 180 0 respectively) PCR SSCP analysis indicated the existence of band shift in exon 7 of KAI1 gene in PC3,PG and PC3M Conclusions Decreased KAI1/CD82 expression may be associated with enhanced metastatic potential in prostate cancer cell lines and lung cancer cell line other than melanoma cell lines The decreased expression may be partially nelaled with mutation of KAI1 gene
出处
《中华医学杂志》
CAS
CSCD
北大核心
1999年第9期708-710,共3页
National Medical Journal of China
