摘要
目的:探讨多氯联苯(PCB)对大鼠睾丸组织表型及表达c-fos、c-Myc和β-catenin的影响。方法:Wistar大鼠45只随机分为4组,对照组饲喂正常饲料,低、中、高剂量组分别饲喂不同PCB含量的颗粒饲料(分别含PCB0.1、1、10mg/kg),90d后建立慢性PCB毒性动物模型。利用组织病理学技术分析PCB对大鼠睾丸组织表型的影响;采用免疫组化方法检测睾丸组织c-fos、c-Myc和β-catenin的表达。结果:PCB染毒组睾丸组织病理学表现为睾丸水肿,间质表型受损,生精小管形态发生改变,间质细胞、初级精母细胞、次级精母细胞消失,精子数量少见。1mg/kg和10mg/kg组c-fos和c-Myc的表达明显强于对照组和0.1mg/kg组(P<0.01);0.1mg/kg组β-catenin蛋白表达下调,与其它各组相比差异显著(P<0.01);1mg/kg组β-catenin的表达高于对照组和10mg/kg组(P<0.05)。结论:PCB能引起大鼠睾丸组织表型的改变,c-fos、c-Myc和β-catenin的异常表达与PCB致睾丸损伤有密切关系。
Objective：To study the effects of polychlorinated biphenyl （PCB） on the phenotype of the testis tissue and the expressions c-fos,c-Myc and β-catenin in the rat testis.Methods： Forty-five Wistar male rats were divided into a control and three experimental groups,the former fed normally,and the latter with PCB at 0.1,1 and 10 mg/kg respectively for 90 days.Then the effects of PCB on the phenotype of the testis tissue and the expressions of c-fos,c-Myc and β-catenin were determined by histopathology and immunohistochemistry.Results： Histopathological examinations revealed testis edema,damage of the mesenchymal phenotype,morphological changes of the contorted seminiferous tubules,absence of stromal cells,spermiocytes and prespermatids,and decreased number of sperm.The expressions of c-fos and c-Myc were significantly higher in the 1 and 10 mg/kg PCB groups than in the control and 0.1 mg/kg PCB groups （P 0.01）.The expression of β-catenin was downregulated in the 0.1 mg/kg PCB group,with significant differences from the other groups （P 0.01）,but it was higher in the 1 mg/kg PCB than in the control and 10 mg/kg PCB groups （P 0.01）.Conclusion： PCB causes changes in the phenotype of the testis tissue,and the abnormal expressions of c-fos,c-Myc and β-catenin are closely related to the PCB-induced testis injury.
出处
《中华男科学杂志》
CAS
CSCD
北大核心
2011年第2期126-130,共5页
National Journal of Andrology
基金
甘肃省自然科学基金(ZR-97089)~~
