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血小板源生长因子A(PDGF—A)在发育性髋关节发育不良关节囊的分布和表达

Expression of platelet-derived growth factor-A in the hip capsule of children with developmental dysplasia of the hip
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摘要 目的探求发育性髋关节发育不良患儿(devdopmentdysptasiaofhip,DDH)和对照组儿童血小板源生长因子A(PIX;FA)在关节囊中的免疫组化分布规律与水平差异,并比较PDGF-A在mRNA水平的表达差异,以探索髋关节松弛的原因。方法共收集了髋脱位患儿64例和对照组儿童14例,二组之间成功配对8组,进行配对研究,每组配对条件为性别相同、年龄相近,采用S-P法免疫组化技术和半定量RT-PCR技术检测关节囊中PDGF-A的分布规律与水平差异,以及在mRNA水平的差异。运用图像分析软件进行量化分析,并采用SPSS10.0软件进行统计学分析。结果可见分泌PDGF—A的成纤维细胞于关节囊内贴近关节侧的滑膜层呈强阳性表达,内部纤维层阳性表达细胞稀疏,强度明显减弱。8例患儿与对照组配对比较,纤维层中有阳性表现的成纤维细胞数占成纤维细胞总数的百分比(配对T检验P〈0.001)和局部染色的灰度值(配对丁检验P〈0.05)在二组间差异存在统计学意义,DDH组关节囊PDGF-AmRNA的表达较对照组减少(配对丁检验P〈0.01)。结论PDGF-A的分布减少和表达异常很可能与DDH患儿的髋关节松弛有关。 Objective To study the expression of platelet-derived growth factor-A (PDGF-A) in the hip capsule of children with developmental dysplasia of the hip (DDH). Methods The clinical data of 64 patients with DDH and 14 control children were retrospectively analyzed. Among them, 8 pairs of DDH patients and their controls were chosen for this study. The tissues were collected during surgery. The PDGF-A in the hip capsule was detected using immunohistochemistry (SP method) staining. RT-PCR was used to study the mRNA expression of PDGF-A. Results High expression of PDGF-A was observed in the synovial layer with fibroblast regularly arranged parallel to the joint sur- face. PDGF-A expression was decreased in the fibrous layer of the hip capsule. The percentage of positive fibroblast and gray-scale density was significantly different in the fibrous layer of the DDH patients compared to controls (P〈0. 05). The mRNA expression of PDGF-A was also decreased in the DDH patients compared to the controls (P〈0. 01 ). Conclusions The decreased expression of PDGF- A in the hip capsule may involve in the development of DDH.
出处 《中华小儿外科杂志》 CSCD 北大核心 2011年第2期124-128,共5页 Chinese Journal of Pediatric Surgery
关键词 髋脱位 发育性 关节不稳定性 关节囊 血小板源生长因子 Hip dislocation,developmental Joint laxity Joint capsule Platelet-derived growth factor
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