摘要
Hydrogen peroxide （H2O2） is a key reactive oxygen species （ROS） in signal transduction pathways leading to activation of plant defenses against biotic and abiotic stresses. In this study, we investigated the effects of H2O2 pretreatment on aluminum （AI） induced antioxidant responses in root tips of two wheat （Triticum aestivum L.） genotypes, Yangmai-5 （AI-sensitive） and Jian-864 （AI-tolerant）. AI increased accumulation of H2O2 and O2^- leading to more predominant lipid peroxidation, programmed cell death and root elongation inhibition in Yangmai-5 than in Jian-864. However, H2O2 pretreatment alleviated AI- induced deleterious effects in both genotypes. Under AI stress, H2O2 pretreatment increased the activities of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and monodehydroascorbate reductase, glutathione reductase and glutathione peroxidase as well as the levels of ascorbate and glutathione more significantly in Yangmai-5 than in Jian-864. Furthermore, H2O2 pretreatment also in- creased the total antioxidant capacity evaluated as the 2, 2-diphenyl-l-picrylhydrazyl-radical scavenging activity and the ferric reducing/antioxidant power more significantly in Yangmai-5 than in Jian-864. Therefore, we conclude that H2O2 pretreatment improves wheat AI acclimation during subsequent AI exposure by enhancing the antioxidant defense capacity, which prevents ROS accumulation, and that the enhancement is greater in the AI-sensitive genotype than in the AI-tolerant genotype.
Hydrogen peroxide （H2O2） is a key reactive oxygen species （ROS） in signal transduction pathways leading to activation of plant defenses against biotic and abiotic stresses. In this study, we investigated the effects of H2O2 pretreatment on aluminum （AI） induced antioxidant responses in root tips of two wheat （Triticum aestivum L.） genotypes, Yangmai-5 （AI-sensitive） and Jian-864 （AI-tolerant）. AI increased accumulation of H2O2 and O2^- leading to more predominant lipid peroxidation, programmed cell death and root elongation inhibition in Yangmai-5 than in Jian-864. However, H2O2 pretreatment alleviated AI- induced deleterious effects in both genotypes. Under AI stress, H2O2 pretreatment increased the activities of superoxide dismutase, catalase, peroxidase, ascorbate peroxidase and monodehydroascorbate reductase, glutathione reductase and glutathione peroxidase as well as the levels of ascorbate and glutathione more significantly in Yangmai-5 than in Jian-864. Furthermore, H2O2 pretreatment also in- creased the total antioxidant capacity evaluated as the 2, 2-diphenyl-l-picrylhydrazyl-radical scavenging activity and the ferric reducing/antioxidant power more significantly in Yangmai-5 than in Jian-864. Therefore, we conclude that H2O2 pretreatment improves wheat AI acclimation during subsequent AI exposure by enhancing the antioxidant defense capacity, which prevents ROS accumulation, and that the enhancement is greater in the AI-sensitive genotype than in the AI-tolerant genotype.
基金
supported by the State Key Basic Research and Development Plan of China (2007CB109305)
National Natural Science Foundation of China (30771292,30900920,30270784)
the Foundation for University PhD.Granting Discipline of the Ministry of Education (20060335014)
The International Plant Nutrition Institute
