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钳夹法造成大鼠视网膜神经节细胞过量丢失 被引量:5

Excessive retinal ganglion cell loss after optic nerve crushed with forceps in rats
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摘要 目的评估钳夹视神经对视网膜神经节细胞(retinal ganglion cells,RGCs)的损伤程度。方法取38只雄性Wistar大鼠,夹持组(n=30)按夹持时间12s、9s、6s、3s、1s分为A-E组,F为反身夹持组,每组各5只大鼠。沿大鼠眼球颞侧暴露视神经,于球后2mm处用90g微型视神经夹夹持视神经,另有40g反向镊在球后2mm处夹持视神经,每只大鼠均左眼手术,右眼正常对照,假手术组(n=8)大鼠左眼仅手术暴露视神经球后段,不予夹持。采用荧光金逆行标记RGCs,视网膜铺片计数,计算RGCs数量及存活率。结果假手术组左右眼RGCs密度相比无显著性差异,细胞密度分别为(2679±67)mm-2、(2689±53)mm-2(P=0.896);夹持组RGCs细胞密度明显下降,分别为(220±167)mm-2、(265±232)mm-2、(298±239)mm-2、(478±682)mm-2、(769±615)mm-2、(974±476)mm-2,夹持冲量(夹持力与时间的乘积)和RGCs存活率呈负相关。结论钳夹法可造成明确、定量的视神经损伤,但损伤量过大、稳定性较差,与临床外伤性视神经病变的发病实际尚有一定差距。 Objective To evaluate the injury degree of retinal ganglion cells(RGCs)after optic nerve crushed with forceps.Methods A total of 38 male Wistar rats were divided into forceps groups,which was subdivided into group A to group E,with crushing time of 12 seconds,9 seconds,6 seconds,3 seconds and 1 second,the group F was the forceps group at reverse direction,5 rats in each group.The optic nerve at temple side of rat eyeball was exposed.Optic nerves at 2 mm behind the eyeball were crushed with 90 g micro forceps and then with the 40 g forceps at reverse direction.Left eye of each rat was taken operation,and right eye was treated as normal control.Left eyes of rats in shame operation group were taken treatment to expose optic nerve behind the eyeball,but not with crush.Amount and survival rate of RGCs were calculated with fluorogold regressive labeling RGCs and retinal stretched preparation.Results Cell densities of left and right eyes of rats in shame operation group were(2 679±67)mm-2 and(2 689±53)mm-2,there was no significant difference(P=0.896),and were(220±167)mm-2,(265±232)mm-2,(298±239)mm-2,(478±682)mm-2,(769±615)mm-2 and(974±476)mm-2 in forceps groups,which were obviously decreased.There was negative correlation between impulse of forceps(force of forceps multiply time)and survival rate of RGCs.Conclusions Crush with forceps can make definite and quantitative optic injury,but the injury is serious and stability is poor.There is still a marked gap between our experiment and the actual pathogenesis of traumatic optic neuropathy.
出处 《眼科新进展》 CAS 北大核心 2011年第2期101-104,共4页 Recent Advances in Ophthalmology
基金 国家自然科学基金资助(编号:30772823)~~
关键词 外伤性视神经病变 视网膜神经节细胞 荧光金 traumatic optic neuropathy retinal ganglion cell fluorogold
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参考文献8

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二级参考文献26

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