期刊文献+

细胞周期素D1、视网膜母细胞瘤样蛋白2及微小染色体维持蛋白7在肝细胞癌中的表达及对预后的意义 被引量:14

Expression and prognostic significance of cyclin D1,RBL2/p130 and MCM7 in hepatocellular carcinoma
下载PDF
导出
摘要 目的:研究细胞周期素D1(cyclin D1)、视网膜母细胞瘤样蛋白2(RBL2/p130)及微小染色体维持蛋白7(MCM7)在肝细胞癌(以下简称肝癌)中的表达及对预后诊断的意义。方法:用免疫组织化学法检测44例肝癌组织、26例癌旁硬化肝组织及18例正常肝组织中cyclin D1、RBL2/p130及MCM7的表达情况,并分析其与肝癌患者临床参数间的关系。结果:肝癌组织中cyclin D1和MCM7的阳性表达率分别是68.2%和72.7%,显著高于正常肝组织及癌旁硬化肝组织(P<0.01);RBL2/p130的阳性表达率为34.1%,显著低于正常肝组织及癌旁硬化肝组织(P<0.01),MCM7与cyclin D1的表达呈正相关(r=0.349,P<0.05),与RBL2/p130的表达呈负相关(r=-0.421,P<0.01);cyclin D1与RBL2/p130的表达呈负相关(r=-0.435,P<0.01)。Cyclin D1及MCM7的表达与肿瘤包膜的完整性、肿瘤分化程度及肝癌临床分期有关,RBL2/p130的表达与有无门脉癌栓、肿瘤分化程度及肝癌临床分期有关(P<0.05)。MCM7还与肿瘤大小及甲胎蛋白(AFP)值的大小有关。多因素分析显示肿瘤大小、MCM7及cyclin D1与肝癌预后相关(P<0.05)。MCM7及cyclin D1阳性表达的患者、RBL2/p130阴性表达的患者预后差(P<0.05)。结论:Cyclin D1、RBL2/p130和MCM7的异常表达在肝癌形成过程中发挥了重要作用。监测其在肝癌中的表达情况将有助于判断肝癌患者的预后。 AIM: To investigate the expression and prognostic significance of cyclin D1, retinoblastoma- like protein 2 ( RBL2/pl30 ) and minichromosome maintenance protein 7 ( MCM7 ) in hepatocellular carcinoma ( HCC ). METHODS: The expression of cyclin D1, RBL2/p130 and MCM7 in 44 HCC specimens, 26 adjacent noncancerous cirrhotic liver specimens and 18 normal liver specimens were detected by the method of immunohistochemistry. The correlations of cyclin D1, RBL2/p130 and MCM7 with the clinical parameters of HCC patients were analyzed. RESULTS: The positive expression rates of cyclin D1 and MCM7 in HCC were 68. 2% and 72. 7%, higher than those in normal livers and adjacent noncancerous cirrhotic livers ( P 〈 0. 01 ). The positive expression rate of RBL2/pl30 in HCC was 34. 1%, lower than that in normal livers and adjacent noncancerous cirrhotic livers(P 〈0. 01 ). The expression of MCM7 in HCC was positively correlated with the expression of cyclin D1 ( r = 0. 349, P 〈 0.05 ), and negatively correlated with the expression of RBL2/p130( r = - 0. 421, P 〈 0. 01 ). The expression of cyclin D1 in HCC was negatively correlated with the expression of RBL2/p130(r = -0. 435, P 〈0. 01 ). The expression of MCM7 and cyclin DI was associated with the integrity of tumor capsule, differentiation degree and TNM stage (P 〈 0. 05 ). The expression of MCM7 was also associated with tumor size and the value of alpha fetoprotein(AFP). The tumor size, the expression levels of MCM7 and cyclin D1 were correlated with the prognosis determined by multivariable analysis. The patients with positive expression of MCM7 and cyclin D1 had lower survival rate than those with negative expression( P 〈 0. 05 ). The patients with positive expression of RBL2/p130 had higher survival rate than those with negative expression (P 〈 0. 05 ). CONCLUSION: The abnormal expression of cyclin D1, RBL2/p130 and MCM7 plays an important role in the development of HCC, indicating that monitoring their expression in HCC patients may be helpful to the judgment of prognosis.
出处 《中国病理生理杂志》 CAS CSCD 北大核心 2011年第2期304-309,共6页 Chinese Journal of Pathophysiology
关键词 细胞周期蛋白D1 视网膜母细胞瘤样蛋白质 微小染色体维持蛋白质 肝肿瘤 Cyelin D1 Retinoblastoma - like protein Miniehromosome maintenance protein Liver neoplasms
  • 相关文献

参考文献13

  • 1Lindner K,Gregn J,Montgomery S,et al.Essential role of MCM proteins in premeiotic DNA replication[J].Mol Biol Cell,2002,13(2):435-444.
  • 2刘娟,姚树坤,殷飞.应用基因芯片筛选人肝癌、肝硬化及正常肝组织中差异表达的基因[J].基础医学与临床,2007,27(1):57-62. 被引量:8
  • 3杨秉辉,夏景林.“原发性肝癌的临床诊断与分期标准”及说明[J].肿瘤,2002,22(1):75-75. 被引量:70
  • 4Ewen ME,Lamb J.The activities of cyclin D1 that drive tumorigenesis[J].Trends Mol Med,2004,10(4):158-162.
  • 5谭伟坚,张晓薇,林仲秋,陈国勤.宫颈上皮内瘤变及宫颈鳞癌中cyclin D1、Ki-67的表达及其意义[J].中国病理生理杂志,2010,26(2):390-392. 被引量:11
  • 6Patil MA,Lee SA,Macias E,et al.Role of cyclin D1 as a mediator of c-Met-and β-catenin-induced hepatocarcinogenesis[J].Cancer Res,2009,69(1):253-261.
  • 7Huynh H.Overexpression of tumour suppressor retinoblastoma 2 protein(pRb2/p130) in hepatocellular carcinoma[J].Carcinogenesis,2004,25(8):1485-1494.
  • 8Claudio PP,Russo G,Kumar CA.pRb2/p130,vascular endothelial growth factor,p27(KIP1),and proliferating cell nuclear antigen expression in hepatocellular carcinoma:their clinical significance[J].Clin Cancer Res,2004,10(10):3509-3517.
  • 9Bell SP,Dutta A.DNA replication in eukaryotic cells[J].Annu Rev Biochem,2002,71:333-374.
  • 10Dudderidge TJ,Stoeber K,Loddo M,et al.MCM2,Geminin,and KI67 define proliferative state and are prognostic markers in renal cell carcinoma[J].Clin Cancer Res,2005,11(7):2510-2517.

二级参考文献21

  • 1周咏梅,李庭芳.CyclinD1和P16在宫颈上皮内瘤变及宫颈鳞癌中的表达及其意义[J].新疆医科大学学报,2005,28(7):658-660. 被引量:7
  • 2Meijer CJ, Snijders PJ, yam den Brule AJ. Screening for cervical cancer: Should we test for infection with high - risk HPV? [J]. CMAJ, 2000,163(5): 535 -538.
  • 3Hunter T, Pines J. Cyclins and cancer Ⅱ: cyclin D and CDK inhibitors come of age [ J ]. Cell, 1994,79 ( 5 ) :573 - 582.
  • 4Bae DS, Cho SB, Kim YJ,et al. Aberrant expression of cyclin D1 is associate with poor prognosis in carly stage cervical of the utcrus[ J I. Gynccol Oncol, 2001,81 (3) : 341 - 347.
  • 5Silva SD, Agostini M, Nishimoto IN, et al. Expression of fatty acid synthase, ErbB2 and Ki -67 in head and neck squamous cell carcinma. A clinicopathological study [ J ]. Oral Oncol, 2004,40 (7) .688 - 696.
  • 6Beenken SW, Bland KI. Biomarkers for breast cancer [J].Minerva Chir,2002,57 ( 4 ) :437 - 448.
  • 7Carreras R, Alameda F, Mancebo G, et al. A study of Ki -67,C- erbB2 and cyclin D1 expression in CIN Ⅰ , CIN Ⅲ and squamous cell carcinoma of the cervix [ J ]. Histol Histopathol, 2007,22 (6) : 587 - 592.
  • 8Carlos ME, Ponce - Castaneda MV, Cee MH, et al. P27KiPl:Chromosomal mapping to 12P12 - 12P13.1 and absence of mutations in human tumor[ J ]. Cancer Res, 1995,55 (6) : 1211 - 1214.
  • 9Pietenpol TA, Bohlander SK, SatoY, et al. Assignment of the human P27KiP1 gene to 12P13 and its analysis in lenkenias [ J ]. Cancer Res, 1995,55 ( 6 ) : 1206 - 1210.
  • 10Parkin DM, Bray F, Ferlay J, et al. Estimating the world cancer burden: Globocan 2000[J]. Int J Cancer, 2001, 94(2) : 153 - 156.

共引文献84

同被引文献178

引证文献14

二级引证文献34

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部