摘要
目的:探讨内毒素血症时大鼠肠组织免疫分子的改变及中药通腑颗粒对其的影响.方法:120只♂Wistar大鼠随机分为正常对照(A)组、内毒素血症(B)组及中药治疗(C)组,各组按造模后预设的取材时间点(2、6、12、24h)分为4小组.通过尾静脉注射的方法建立内毒素血症模型,采用酶联免疫吸附(ELISA)法及放射免疫分析的方法,检测肠组织中的免疫分子及肠内容物中的分泌性免疫球蛋白(sIgA).结果:A、C组INF-γ含量在24h明显高于B组(32.93ng/L±13.17ng/L,37.14ng/L±6.70ng/Lvs23.21ng/L±8.65ng/L,均P<0.05),C组INF-γ含量在12h明显高于A组(39.96ng/L±8.26ng/Lvs29.64ng/L±10.78ng/L,P<0.05).A、C组IL-4含量在6h明显高于B组(115.56ng/L±17.67ng/L,124.39ng/L±26.02ng/Lvs83.36ng/L±27.24ng/L,P<0.05或0.01),但B组IL-4含量在24h较A组明显升高(107.96ng/L±10.86ng/Lvs92.21ng/L±17.62ng/L,P<0.05).B组Foxp3含量在12、24h较A组明显增加(0.28g/L±0.037g/Lvs0.24g/L±0.041g/L;0.26g/L±0.029g/Lvs0.22g/L±0.041g/L,均P<0.05),C组Foxp3含量24h明显低于B组(0.23g/L±0.030g/Lvs0.26g/L±0.029g/L,P<0.05).A、C组sIgA含量2、12、24h明显高于B组(2.43×10-11±0.23×10-11,2.18×10-11±0.27×10-11vs1.66×10-11±0.25×10-11;2.76×10-11±0.22×10-11,2.64×10-11±0.22×10-11vs2.09×10-11±0.20×10-11;2.48×10-11±0.31×10-11,2.25×10-11±0.44×10-11vs1.88×10-11±0.45×10-11,均P<0.05),C组与A组进行对比在2h明显降低.结论:内毒素血症时肠黏膜损伤,肠道产生sIgA减少,肠组织中抑炎因子IL-4先减少后增多,Foxp3增多,而促炎因子INF-γ减少,肠免疫功能发生由炎症反应向抗炎反应的转变,免疫功能出现抑制.应用中药通腑颗粒可以减轻肠黏膜损伤,双向调节炎症反应,保护肠免疫功能.
To observe the changes in the levels of intestinal cytokines in rats with endotoxemia and to investigate the effects of treatment with Tongfu Granules on intestinal cytokine levels. METHODS： One hundred and twenty male Wistar rats were randomly divided into three groups： control group, endotoxemia group and treatment group. Endotoxemia was induced bya single injection of LPS via the caudal vein. The levels of intestinal cytokines were measured by ELISA and radioimmunoassay. RESULTS： The level of IFN-y at 24 h was significantly lower in the endotoxemia group than in the control and treatment groups （32.93 ng/L ± 13.17 ng/L, 37.14 ng/L ± 6.70 ng/L vs 23.21 ng/L ± 8.65 ng/L, both P 〈 0.05）. The level of IFN-γ at 12 h was significantly higher in the treatment group than in the control group （39.96 ng/L ± 8.26 ng/L vs 29.64 ng/L ±10.78 rig/L, P 〈 0.05）. The level of IL-4 significantly decreased in the endotoxemia group at 6 h but increased at 24 h compared with the control and treatment groups （115.56 ng/L ＋ 17.67 ng/L, 124.39 ng/L ± 26.02 ng/L vs 83.36 ng/L ± 27.24 ng/L, both P 〈 0.05 or 0.01）. IL-4 level at 24 h was significantly higher in the endotoxemia group than in the control group （107.96 ng/L ＋ 10.86 ng/L vs 92.21 ng/L ± 17.62 ng/L, P 〈 0.05）. The levels of Foxp3 at 12 and 24 h were significantly higher in the endotoxemia group than in the control group （0.28 g/L ± 0.037 g/L vs 0.24 g/L ± 0.041 g/L; 0.26 g/L ±0.029 g/L vs 0.22 g/L ± 0.041 g/L, both P 〈 0.05）. The level of Foxp3 at 24 h was signifi- cantly lower in the treatment group than in the endotoxemia group （0.23 g/L ± 0.030 g/L vs 0.26 g/L ± 0.029 g/L, P 〈 0.05）. The levels of slgA at 2, 12 and 24 h was significantly lower in the endo- toxemia group than in the control and treatment groups （2.43 x 10-11 ± 0.23 x 10-11, 2.18 x 10-11 ± 0.27 x 10-11 vs 1.66 x 10-11 ± 0.25 x 10-11; 2.76 x 10-11 ± 0.22 x 10-11, 2.64 x 10-11 ± 0.22 x 10-11 VS 2.09 X 1011 ± 0.20 × 10-11; 2.48 x 1011 ＋ 0.31 x 10-11, 2.25 x 10-11 ＋ 0.44 X 10-11 VS 1.88 X 10-11 ＋ 0.45 X 10% all P 〈 0.05）. The level of sIgA at 2 h was significantly lower in the treatment group than in the control group. CONCLUSION： Small intestinal mucosa injury and deregulated inflammatory reaction can result in intestinal immunodepression in rats with endotoxemia. Treatment with Tongfu Granules can protect intestinal immune function in rats with endotoxemia by reducing small intestinal mucosa injury and bidirectionally regulating inflammatory reaction.
出处
《世界华人消化杂志》
CAS
北大核心
2010年第33期3515-3519,共5页
World Chinese Journal of Digestology
