摘要
目的 观察辛伐他汀在体内外对载脂蛋白M（apoM）的影响.方法 脂肪乳灌胃建立昆明小鼠高脂血症模型,给予辛伐他汀（10、100 mg/kg）连续灌胃6周.酶法检测胆固醇等血脂水平,Dot Blot分析血清apoM水平,逆转录-聚合酶链反应（RT-PCR）检测HepG2细胞中apoM mRNA水平.结果 与模型对照组比较,100mg/kg辛伐他汀组小鼠血清胆固醇水平明显升高（P＜0.05）.26周龄小鼠与12周龄比较,4组血清apoM水平均随鼠龄增加而降低（P＜0.05）.26周龄时各组之间apoM水平的差异无统计学意义（P＞0.05）.10 μmol/L辛伐他汀组HepG2细胞apoM mRNA水平较对照组低51.93%（P＜0.01）.结论 辛伐他汀对高脂血症小鼠血清apoM水平无明显影响,鼠龄可能是影响昆明小鼠apoM的因素.
Objective To investigate the effects of simvastatin on apolipoprotein M （apoM） in vivo and in vitro.Methods The 12-weeks-old Kunming male mice （ clean animal） were randomly divided into two groups,and given intragastric administration with 0.9% saline （ control）,lipid emulsion （ high fat） at the daily dose of 15 ml/kg respectively.After 8 weeks the hyperlipidemic models were successfully induced and randomly divided into three groups：high fat model control group,high-dose simvastatin-treated group,and regular simvastatin-treated group.Mice were dosed daily for 6 weeks with simvastatin （ 10 or 100 mg/kg body weight） at 20 weeks of age.Mice were sacrificed for examination of serum lipids and the protein levels of apoM in mice serum were estimated by dot blotting analysis.In vitro,apeM mRNA expression in HepG2 cells treated with simvastatin was determined by real-time reverse transcription-polymerase chain reaction （RT-PCR）.Results As compared with high fat model control group,100 mg/kg simvastatin significantly increased serum total cholesterol （ P〈0.05 ）.At the age of 26 weeks,the serum apoM levels were significantly decreased （ P〈0.05 ） in normal control group,high fat model control group,regular simvastatin-treated group,and high-dose simvastatin-treated group as compared with those at the age of 12 weeks.However,at the age of 26 weeks vs 20 weeks only in normal control group the serum apoM levels were decreased by 17.20% （P〈0.05 ）,and the remaining were modestly reduced but had no statistically significant difference （P 〉0.05）.Neither 10 mg/kg nor 100 mg/kg simvastatin had effect on serum apoM levels as compared with high fat model control group （P 〉0.05）.In vitro,apoM mRNA levels in 10 μmol/L simvastatin-treated group was reduced by 51.93% as compared with control group in HepG2 cells （P〈0.01 ）.Conclusion APoM levels were decreased with the increase of age in normal mice.It was likely that age may therefore be of importance for regulation of apoM in Kunming mice.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第3期442-445,共4页
Chinese Journal of Experimental Surgery
基金
基金项目：国家自然科学基金资助项目（30570752、30972955）
江苏省自然科学基金资助项目（BK2008140）
江苏省卫生厅面上资助项目（H200820）
江苏省常州市社会发展资助项目（CS2008201）
关键词
辛伐他汀
高脂血症
载脂蛋白
Simvastatin
Hyperlipidemia
Apolipoprotein
