摘要
以苜蓿（Medicago sativa）多叶性状S1代的3个单株为材料,研究了3份参试材料的败育率,测定了开展胚挽救培养的最佳取材时间和不同激素水平对苜蓿自交系胚挽救的影响。结果表明,参试材料平均败育率为37.59%;进行苜蓿胚挽救培养的最佳取样时间为授粉后30 d;胚挽救培养的最佳培养基配方为MS＋0.5 mg/LIAA＋0.07 mg/L 6-BA,蔗糖30 g/L,琼脂7 g/L,pH值5.8~6.1。对萌动的胚进行愈伤组织诱导发现,参试材料平均诱导率为85.32%,愈伤组织平均分化率为75.66%。研究以期为苜蓿胚挽救育种提供理论基础,并为苜蓿特异性状自交系结实率低等方面的研究提供有效的解决途径。
Three alfalfa（Medicago sativa） plants of selfing S1 generation with multifolate characteristics were used to study embryo abortion rate of alfalfa inbred lines and effect of best sampling time and different levels of hormone on embryo rescue.The results pf this study showed that the average abortion rate of embryos was 37.59%.The best sampling time of embryo rescue culture was 30 d after pollination.The optimum medium of embryo rescue culture was MS medium containing 0.5 mg/L IAA and 0.07 mg/L 6-BA＋sucrose 30 g/L＋agar 7 g/L,and pH 5.8-6.1.The average induction and differentiation rate of embryogenic callus were 85.32% and 75.66%,respectively.This study provided a theoretical basis of embryo rescue and an effective solution of low selfing fruitful rate of multifoliate alfalfa.
出处
《草业科学》
CAS
CSCD
北大核心
2011年第2期226-230,共5页
Pratacultural Science
基金
国家863计划项目"高产
多抗
优质苜蓿新品种分子聚合育种"(2008AA10Z149)
国家自然科学基金项目"苜蓿多叶性状的遗传特性和基因组学研究"(30972136)
关键词
苜蓿
胚挽救
自交
败育率
alfalfa（Medicago sativa）
embryo rescue
selfing
abortion rate
