摘要
目的对Western Blot和流式细胞术(Flowcytometry,FCM)这两种磷酸化STAT3的检测方法进行比较。方法用不同浓度的白细胞介素5(interleukin 5,IL-5)刺激人早幼粒细胞白血病细胞株HL-60,活化细胞内的STAT3,然后分别用Western Blot和流式细胞术进行检测。结果 Western Blot与流式细胞术的检测结果一致。结论 Western Blot与流式细胞术用于磷酸化STAT3的检测各有优势,可以互为补充。
Objective FCM（flow cytometry） is compared with Western blot in investigating the phosphorylated of STAT3.Methods Promyelocytic leukemia cell line HL-60 are cultured and stimulate with human IL（hIL）-5 in different concentration and then are detected with Western Blot and FCM simultaneously.Results The results of Western blot and FCM are identical.Conclusion The two methods are all have their own advantages in investigating phosphorylated STAT3 and they are mutually complementary.
出处
《中国实验诊断学》
北大核心
2011年第2期209-211,共3页
Chinese Journal of Laboratory Diagnosis
基金
四川省卫生厅课题(编号:000183)
