摘要
目的研究临床分离大肠埃希菌的耐药机制及遗传多态性。方法用WHONET5.4分析菌株药敏情况。PCR检测大肠埃希菌I类整合酶基因、插入序列共同区(ISCR/orf513)和产超广谱β-内酰胺酶(ESBLs)基因;以肠杆菌科间的重复序列(ERIC)-PCR检测基因型,SPSS分析多态性。结果除亚胺培南、美洛培南、四环素和哌拉西林/他唑巴坦,产ESBLs菌的耐药率明显高于不产ESBLs菌(P<0.05)。72株产ESBLs株中I类整合酶,ISCR,blaTEM,blaSHV,blaCTX-M的阳性率分别为51.4%,12.5%,80.6%,0%和36.1%;而27株不产ESBLs株中的阳性率分别为37.0%,3.7%,81.5%,3.7%和3.7%。根据指纹图谱把99株大肠埃希菌基因型分为79种,经SPSS系统聚类分析,可归为18个大泪。结论我院产ESBLs大肠埃希菌主要是CTX-M型;产ESBLs株的I类整合酶阳性率明显高于不产ESBLs株,整合子和ISCR可以共存;ERIC-PCR是一种效果较好的基因分型方法,我院大肠埃希菌散发存在。
Objective To study the mechanism of drug resistance and the homology among Escherichia coli clinical isolates.Methods Analyze the resistant rates by WHONET5.4.Genes of Class I integrase,ISCR and ESBLs were detected by PCR.The homology was tested by enterobacterial repetitive intergenic consensus PCR（ERIC-PCR）,and analyzed by SPSS.Results The resistant rates of the producing ESBLs ones were significantly higher than those of non-producing ones（P0.05）,excluding Imipenem、Meropenem、Tetracycline and Piperacillin/Tazobactam.Among 72 strains producing ESBLs,the positive rates of Class I integrase,ISCR and ESBLs（blaTEM,blaSHV,blaCTX-M）were 51.4%,12.5%,80.6%,0% and 36.1%,respectively.However,among 27 non-ESBLs strains,the positive rates were 37.0%,3.7%,81.5%,3.7% and 3.7%,respectively.There were 79 kinds of genotype.99 isolates were divided into 18 groups according to the analysis by SPPP.Conclusion CTX-type is predominant genotype of ESBL in our hospital;The positive rate of Class I integrase of the producing ESBLs ones is significantly higher than those of non-producing ones.Integron can exist with ISCR;ERIC-PCR is a useful method for the molecular epidemiology of Escherichia coli isolates,there are no nosocomial infection of Escherichia coli in our hospital.
出处
《中国实验诊断学》
北大核心
2011年第2期293-297,共5页
Chinese Journal of Laboratory Diagnosis
