摘要
本文用凝血酶亲和层析,制备电泳分离纯化了兔肺血栓调节蛋白(TM)。并对兔肺TM抑制血凝和血小板聚集,以及激活蛋白C 等作用进行了初步研究。结果表明,兔肺TM 的抗凝作用主要是抑制了内源性凝血系统,而对外源性凝血系统影响很小;并可通过蛋白C 系统起抗凝和纤溶作用;能完全抑制由凝血酶和瑞斯托霉素诱导的血小板聚集。
Rabbit Thrombomodulin was isolated from lung by triton X 100 extraction,purified by affinity chromatography on PMSF-thrombin-Sepharose 4 B column andpreparative electrophoresis.The effect of rabbit lung thrombodulin on anticoagula-tion was investigated.It shows that thrombomodulin not only accelerates the rateof protein C activation caused by thrombin,but also inhibites thrombin-inducedhuman plasma clotting.Additionally,thrombomodulin can entirely inhibit throm-bin and ristocetin-induced platelet aggregation.
出处
《苏州医学院学报》
1990年第3期188-191,254-260,共4页
Acta Academiae Medicinae Suzhou
关键词
血栓调节蛋白
分离
纯化
抗凝作用
thrombomdulin
blood coagulaion
platelet aggregation
anticoagulation
