摘要
绵麦冬经乙醇除脂后,用超声波辅助法进行提取,再经三氯乙酸除蛋白,乙醇沉淀后得粗多糖(CP)。用DEAE-Sepharose Fast Flow离子交换柱色谱分离纯化粗多糖,并进行光谱学分析和自由基清除活性探讨。结果显示:经离子交换柱首次分离出1种中性糖(NP)和3种酸性糖(AP)。中性糖的1,1-二苯基-2-三硝基苯肼自由基(DPPH·)和羟自由基清除(·OH)能力都不强;3种AP的DPPH·和·OH清除能力为AP3>AP2>AP1。AP3具有极高的.OH清除能力。粗多糖的·OH清除能力随浓度变化大于AP1和AP2,DPPH·清除能力显著高于纯化后的多糖。各糖对DPPH·清除能力的半数抑制质量浓度(IC50)分别为:AP1 22.30 g/L,AP2 9.96 g/L和AP3 7.70 g/L,CP 2.01 g/L;对·OH清除能力的IC50分别为:AP1 8.42 g/L、AP225.73 g/L和AP3 1.86 g/L,CP 4.93 g/L。
Crude polysaccharides （CP） were obtained from Ophiopogon japonicus （ Thumb. ） Ker-Gawl by ultrasonic extraction, followed by trichloracetic acid deprotelnation and ethanol precipitation. CP were further purified by DEAE-sepharose fast-flow ionexchange column chromatography. Finally the spectroscopy of CP was analyzed and the free radical scavenging activities of various fractions toward DPPH＂ and ·OH were studied. One neutral polysaccharide（NP） and three kinds of acidic polysaccha- rides（AP） were separated by DEAE-sepharose FF. The result showed that NP has very low activities in scavenging DPPH. and ·OH. The ability of three kinds of AP for scavenging DPPH· and ·OH were in the sequence of： AP3 〉 AP2 〉 AP1. AP3 has high ability of scavenging .OH . CP has very high ability of scavenging DPPH. , and its ability of scavenging .OH changed rapidly with the concentration change. The ICso of various polysaccharides toward DPPH＂ were： AP1 22.30 g/L, AP2 9.96 g/L, AP3 7.70 g/ L; CP 2.01 g/L. The IC50 of various polysaceharides toward .OH were： APl 8.42 g/L, AP2 5.73 g/L, AP3 1.86 ~./ L： CP 4.93 g./L.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2011年第1期68-72,共5页
Chemistry and Industry of Forest Products
基金
西南科技大学博士基金项目(06zx7122)
关键词
绵麦冬
多糖
分离纯化
自由基清除活性
Ophiopogons japonicus （ Thumb. ） Ker-Gawl
polysaccharides
extraction and purification
free radical scavengingactivity
