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不同加工工艺对大豆转基因成分及调控元件的影响 被引量:7

Effect of Different Processing on Transgenic Ingredients and Regulatory Elements of Soybean
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摘要 以转基因大豆为试验材料,运用定性PCR的方法研究了粉碎、湿热和膨化3种加工工艺对转基因大豆内源基因Lectin、外源基因Cp4epsps以及启动子和终止子的影响。结果表明:大豆经过100℃湿热处理15 min后,内源基因降解到407 bp以下;经过100℃湿热处理15 min后,外源基因降解到408 bp以下;在100℃经3 min湿热处理后启动子和终止子都产生了降解。机械粉碎与液氮粉碎都未能使大豆基因产生降解,而膨化工艺使大豆内源基因降解到836 bp以下,外源基因降解到1 512 bp以下,但启动子与终止子并未产生降解。因此,粉碎、湿热和膨化加工工艺对大豆转基因成分及调控元件的降解都有不同程度的影响。 Based on generally modified soybeans as materials,used the qualitative research methods of PCR to study the effects to Lectin gene and exogenous gene of Cp4 epsps and promoter and terminator by crushing and sticky-heating and extrusion processing.Researches showed that endogenous gene degraded below to 407 bp through processing at 100℃ sticky-heating for 15 min.Exogenous gene degraded below to 408 bp after 100℃ sticky-heating for 15 min.Promoter and terminator turn to degrade by 100℃ sticky-heating for 3 min.The results also indicated that gene sections had no changes through mechanical disintegration and liquid nitrogen processings.While promoter and terminator showd no degrades through extrusion processing,on the contrary,endogenous gene degraded below to 836 bp and exogenous gene degraded below to 1 512 bp.
出处 《大豆科学》 CAS CSCD 北大核心 2011年第1期136-140,共5页 Soybean Science
基金 转基因生物新品种培育重大专项资助项目(2009ZX08012-012B)
关键词 大豆 加工工艺 内源基因 外源基因 启动子 终止子 PCR技术 Soybean Processing Endogenous gene Exogenous gene Promoter Terminator PCR technology
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参考文献7

  • 1郑文杰,刘烜,刘伟,唐丹舟,何霖,张宏伟.转基因大豆加工产品的定性PCR检测[J].农业生物技术学报,2003,11(5):467-471. 被引量:40
  • 2朱元招,尹靖东,李德发,王凤来.抗草甘膦转基因大豆PCR定量检测研究[J].中国农业大学学报,2005,10(3):25-29. 被引量:27
  • 3Hagen M.Detection of genetically modified soy(Roundup-Ready)in processed food products[J].Berl Munch Tierarztl Wochenschr,2000,113(11-12):454-458.
  • 4GawienowskiM C,Eckhoff S R,Ping Y,et al.Fate of maize DNA during steeping wet milling and processing[J].Cereal Chemistry,1999,76(3):371-374.
  • 5Kharazmi M,Bauer T,Hammes W P,et al.Effect of food processing on the fate of DNA with regard to degradation and transforms-tion capability in Bacillus subtilis[J].Systematic and Applied Microbiology,2003,26(4):495-501.
  • 6Chiter A,Michael J,Forbes M,et al.DNA stability in plant tissues:implications for the possible transfer of genes from genetically modified food[J].Federation of European Biochemical Societies Letters,2000,481:164-168.
  • 7陈颖,王媛,徐宝梁,葛毅强.Roundup Ready~大豆外源基因在食品加工过程中的降解变化[J].中国油料作物学报,2005,27(2):10-14. 被引量:10

二级参考文献37

  • 1Sambrook JFritsch E FE F Maniatis T.金冬雁黎孟枫(translation).[A]..Molecular Cloning: 2nd ed[C].Beijing: Science Press,1996.672-681.
  • 2Frederick M Ausvbel颜子颖 王海环 (translation).Short Protocols in Molecular Blilogy:3rd ed[M].Beijing: Science Press,1998.37.
  • 3James C. The global review of commercialized transgenic crops. ISAAA (International Service for the Acquisition of Agribiotech Applications) Briefs, 2000, 21:1-2.
  • 4Corie L. Americans perplexed by GM food. Nature, 2001,410:501.
  • 5Jayaraman K S. A sound approach to GM debate. Nature,2001, 412:569.
  • 6Bowles D, Klee H. Introduction to the special issues on plant GM technology: Gmspecial issue. Plant J, 2001, 27:481-482.
  • 7Wolfenbarger L L, Phifer P IL The ecological risks and benefits of genetically engineered plants. Science, 2000, 290:2088-2093.
  • 8Hupfer C, Hotzel H, Sachse K, et al. Detection of the genetically modification in heat treated products of Bt maize by polymerase chain reaction. Z Lebensm Unters-Forsch,1999, 206:203-207.
  • 9Hemmer W. Food derived from geneticaUy modified organisms and detection methods. BATS Rep, 1997, 2:1-3.
  • 10Meyer R, Chardonnens F, Hubner P, et al. Polymerase chain reaction in the quality and safety assurance of food:detection of soya in processed meat products. Z Lebensm Unters Forsch, 1996, 203:339-344.

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