摘要
通过重氮化法引入羧基,再用碳二亚胺法偶联BSA获得己烯雌酚(DES)免疫抗原。经紫外光谱、电泳图谱分析确证获得DES-BSA偶联物。间接酶联免疫法分析证明其免疫鼠所获得的抗体与己烯雌酚有良好的特异性。
The immunogen DES-BSA（diethylstibestrol-bovine serum albumin） were synthesized by the carbodilimide method through introduction of a carboxyl group into diethylstilbestrol by diazotization method.The results from UV,SDS-PAGE and ELISA showed that DES-BSA were conjugated successfully.The indirect ELISA results showed that antibodies derived from mice were specific to diethylstilbestrol.
出处
《动物医学进展》
CSCD
北大核心
2011年第2期29-32,共4页
Progress In Veterinary Medicine
基金
国家863高新技术发展计划课题(2008AA10Z425)
江苏省科技支撑计划(BE2008631)
无锡市科技支撑计划(CSE00804)
关键词
己烯雌酚
人工抗原
重氮化
碳二亚胺法
diethylstilbestrol
artificial antigen
diazotization
carbodiimide method
