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蛋白酶体抑制剂MG132抑制体外培养心肌细胞肥大的实验研究

Proteasome inhibitor MG132 attenuate cardiomyocyte hypertrophy in vitro
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摘要 目的研究蛋白酶体抑制剂MG132对体外培养的大鼠心肌细胞肥大的影响。方法采用[3H]-亮氨酸参入率测定心肌细胞蛋白质合成代谢,采用IPP 6.0图像分析软件分析心肌细胞表面积,以及用RT-PCR检测β-肌球蛋白重链(β-MHC)表达。结果 MG132预处理逆转了苯肾上腺素诱导的心肌细胞[3H]-亮氨酸参入率增加(P<0.01)、表面积增大(P<0.01)和β-MHC mRNA表达上调(P<0.05)。结论 MG132能够抑制心肌细胞肥大,具有心肌保护作用。 Objective To investigate the effects of proteasome inhibitor MG132 on cardiomyocyte hypertrophy in vitro.Methods Cardiomyocyte protein synthesize was determined by measuring the-Leucine incorporation rate.With the aid of IPP 6.0 Image software,the surface areas of cardiomyocytes was analyzed.The mRNA expression of fetal gene β-myosin heavy chains（β-MHC） was measured by reverse transcription-polymerase chain reaction（RT-PCR）.Results MG132 significantly inhibited phenylephrine（PE） induced increase in cardiomyocyte-Leucine incorporation rate（P0.01）,surface area（P0.01）and β-MHC mRNA expression（P0.05）.Conclusion MG132 may inhibite cardiomyocyte hypertrophy,and demonstrate an cardioprotective activity.
作者 朱静雯 陈保林 马跃东 刘晨
机构地区 贵州省人民医院干医科 贵州省人民医院心内科 中山大学附属第一医院心血管医学部 中山大学卫生部辅助循环重点实验室
出处 《重庆医学》 CAS CSCD 北大核心 2011年第6期532-534,共3页 Chongqing medicine
关键词 心肌细胞 肥大 蛋白酶体 Β-肌球蛋白重链 cardiomyocyte hypertrophy proteasome β-myosin heary chains
分类号 R542.2 [医药卫生—心血管疾病]
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参考文献17

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重庆医学
2011年 第6期

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