摘要
为了考察光暗对比以及不同碳源类型和浓度对于三角褐指藻生化物质的影响,用含有葡萄糖、乙酸钠和甘油的培养基对三角褐指藻(Phaeodactylum tricornutum)进行培养,并测定生物量、细胞浓度、生化物质以及脂肪酸含量。结果表明,三角褐指藻具有光自养和兼养生长的能力;三角褐指藻对底物浓度与有机碳源具有选择性,其中利用葡萄糖的最佳浓度为20mmol/L;在500mL三角瓶培养过程中生长适宜条件为:温度(25±1)℃,光强25μmol/(m2.s),pH为7.5。不管哪种碳源均能够促进三角褐指藻生物量的积累。将细胞浓度达到2.5×106个/mL培养末期的藻液进行蛋白质、还原糖、叶绿素以及总脂(TOL)含量分析,考察不同生长条件下生化物质的积累以及细胞生物量的变化。通过超声波萃取及索氏抽提总脂,质量分数达到20%(干重);利用GC-MS分析脂肪酸含量及组成,其中以C16∶1,C16∶0居多,质量分数分别为34.8%,11.9%,可以用于提取生物柴油。
In order to understand the effect of light,carbon sources and concentration on the phaeodactylum tricornutum,the culture medium containing glucose,acetate and glycerol were chose,the biomass,cell concentration,biochemical substances and fatty acid content were tested.The results show that the phaeodactylum tricornutum is photoautotroph and mixotroph,which with selectivity for substrate concentration and organic carbon sources,the optimum concentration of glucose is 20 mmol/L,the optimal growth condition in 500 mL flask contains that the temperature is（25±1） ℃,the light intensity is 50 μmol/（m2·s）,the pH is 7.5.Whatever the carbon sources are able to promote the biomass accumulation.When the cell concentration achieve to 2.5×106 cells/mL in the evening of the culture,the protein,sugar,chlorophyll and TOL content were analyzed.The biomass accumulation and biomass change in different growth conditions were determined.By ultrasonic extraction and Soxhelt extraction,the total of lipid reaches 20%（dry weight）;The fatty acid content and composition with GC-MS were measured,and found the C16∶0 and C16∶1 are 34.8% and 11.9%（mass fraction） respectively,which are more than other components and could be used to extract bio-diesel.
出处
《石油化工高等学校学报》
CAS
2011年第1期1-5,共5页
Journal of Petrochemical Universities
基金
国家973科技计划项目资助(S2010021001)
关键词
三角褐指藻
有机碳源
光暗对比
葡萄糖
生物量
总脂含量
生物柴油
Phaeodactylum tricornutum
Organic carbon source
Light/darkness
Glucose
Biomass
Total liquid
Biodiesel
