摘要
为了建立猪捷申病毒(PTV)的血清学检测方法,试验根据GenBank中已经登录的PTV基因序列设计了针对VP1基因的引物,将VP1基因经PCR扩增后克隆到真核表达载体pCAGGS中,用重组质粒pCAGGS-VP1转染293T细胞。结果表明:经间接免疫荧光方法验证VP1获得了表达;利用所建立的PTV VP1表达系统对哈尔滨周边地区的575份猪血清样品进行检测,PTV感染的阳性率为60.17%,说明哈尔滨周边地区猪群对PTV的感染率较高。
To develop a serological detection system for porcine teschovirus （PTV）, a pair of primers targeting PTV VP1 gene was designed based on the GenBank published. VP1 gene was amplified by RT -PCR and inserted into the eukaryotie expression vector pCAGGS. The vec- tors carrying PIN VP1 gene were transfected into 293T cells, this VP1 expression system was used to detect antibodies against PTV in serum of pigs by using indirect immunofluorescence assay. A total of 575 serum samples from different regions near Harbin were tested. The results showed that the positive rate of PTV antibody was 60.17%, suggesting high infection rate in pigs. The serological detection system developed in this study can be used as an epidemiological surveillance tool for Porcine teschovirus.
出处
《黑龙江畜牧兽医》
CAS
北大核心
2011年第2期14-17,共4页
Heilongjiang Animal Science And veterinary Medicine
基金
"十一五"国家科技支撑计划项目(2006BAD06A18)
