摘要
目的:研究中药提取物CTE对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)所致帕金森病小鼠模型是否具有神经保护作用。方法:首先建立MPTP所致帕金森病小鼠模型,连续4 d给予MPTP(30 mg.kg-1,qd,ip)。用高低剂量(50和100 mg.kg-1,qd,ig)中药提取物CTE预处理MPTP所致帕金森模型小鼠。之后进行行为学检测,包括一般行为学检测和滚筒实验。最后,用HPLC-EC法测定纹状体中多巴胺(DA)及其代谢产物二羟苯乙酸(DOPAC)和高香草酸(HVA)含量。结果:在MPTP所致的帕金森病小鼠模型,中药提取物CTE能显著改善小鼠的行为能力;且能显著提高小鼠纹状体内多巴胺含量。结论:中药提取物CTE对MPTP所致帕金森病小鼠模型有神经保护作用。
Objective： To investigate the neuroprotective effect of CTE,an extract from traditional Chinese medicine,in a mouse model of Parkinson＇s disease induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine（MPTP）.Methods： We induced Parkinson＇s disease model by MPTP（30 mg·kg-1,qd,ig）.After pretreatment with high and low doses（50 and 100 mg·kg-1,qd,ig） of CTE,behavioral changes were tested after MPTP induction.The content of dopamine and its metabolites（DOPAC and HVA） were determined by HPLC-EC.Results： In the mouse model of MPTP-induced Parkinson＇s disease,CTE significantly improved the behavior dysfunctions,and significantly increased levels of dopamine in the striatum.Conclusion： The traditional Chinese medicine extract CTE has neuroprotective effect in the mouse model of MPTP-induced Parkinson＇s disease.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2011年第4期355-358,共4页
Chinese Journal of New Drugs
基金
国家自然科学基金(30973889)
