摘要
目的观察昆明小鼠补充不同剂量碘酸钾(KIO3)对肝脏抗氧化能力的影响。方法将160只昆明小鼠随机分为4个剂量组:适碘组(NI),5倍高碘组(5HI),10倍高碘组(10HI),50倍高碘组(50HI);每组再分为雌雄两个组。各组动物均饲以正常鼠饲料(平均碘含量为300μg/kg),NI组饮用去离子水,各HI组分别饮用含不同浓度(2 023.6、4 553.2、24 789.6μg/L)碘酸钾的去离子水,每只小鼠每日的总摄碘量依次为1.5、7.5、15、75μg。喂养3和6个月后,检测肝组织匀浆中的谷胱甘肽过氧化酶(GPx)活力、超氧化物歧化酶(SOD)活力以及丙二醛(MDA)含量。结果不同剂量组之间比较,10HI组和50HI组小鼠肝脏的GPx活力在3和6个月时均较同性别的NI组增高,差异有统计学意义(P<0.05),而SOD活力在6个月时较NI组降低,差异有统计学意义(P<0.05);不同性别比较发现,10HI组和50HI组的雌性组的GPx活力较雄性组有显著增高,差异有统计学意义(P<0.05)。3个HI组的MDA含量与NI组比较在3和6个月时差异均无统计学意义。结论高碘导致小鼠肝脏抗氧化酶GPx活力增高和SOD活力降低,而且雌性组GPx活力增高较雄性组更明显,但无论是雄性组还是雌性组均未见高碘对肝脏产生明显过氧化损伤。
Objective To study the effect of different high dosage KIO3 on the anti-oxidative capability of the liver in mice. Methods The Kunming mice were randomly divided into 8 groups and given KIO3 of different dosage, those were normal iodide （NI）, 5 fold high iodide （5HI）, 10 fold high iodide （10HI） and 50 fold high iodide （50HI）, furthermore, each group were divided into two groups, male and female. By controlling foodstuff and drinking water （adding different amounts of potassium iodate into water）, all groups got prospective iodine intake respectively, that is, 1.5μg/d, 7.5 μg/d, 15 μg/d and 75 μg/d. 3 and 6 months later, the mice were sacrificed and the activities of GPx, SOD and MDA content in the liver were measured. Results The GPx activities of 10HI and 50HI groups were higher than that of NI group after administration for 3 and 6 months, but no difference was found in 5HI group. The SOD activities of 10HI and 50HI groups were lower than that of NI group after administration for 6 months. There were no difference in MDA content between NI group and 3 high iodide groups. Furthermore, The GPx activity of female mice was higher than that of male mice. Conclusion High iodide intake can decrease the anti-oxidative capability of liver in normal mice, especially in female, but no obvious oxidative damage has been found in the liver after a long period of administration by high dosage potassium iodate.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2011年第1期17-19,共3页
Journal of Environment and Health
基金
河南省博士后科研启动基金
国家自然科学基金重点项目(30230330)
关键词
碘酸钾
抗氧化能力
肝脏
雌激素
小鼠
Potassium iodate
Anti-oxidative capacity
Liver
Estrogen
Mice
