摘要
以蛋白的提取率为指标,利用Alcalace、果胶酶和纤维素酶在缓冲液条件下对茶树修剪叶中蛋白的提取进行了研究,发现这三种酶均能有效提高蛋白质的提取率,利用果胶酶和纤维素酶分别与Alcalace合用发现,纤维素酶和Alcalace合用能够很明显提高蛋白质的提取率,比单用Alcalace或纤维素酶分别提高了近35%和近50%。对利用Alcalace和纤维素酶协同作用得到的茶叶蛋白进行分析发现,利用碱法和酶法提取对于蛋白质的氨基酸组成基本没有影响,除吸水性和起泡稳定性略差于碱提茶叶蛋白外,酶法提取茶叶蛋白的其它指标如吸油性、乳化性、乳化稳定性以及起泡性均优于碱提茶叶蛋白。
Protein was extracted from pruned tea leaves by Alcalace,pectinase,and cellulase,respectively. The yield increased significantly when the Alcalace,pectinase,and cellulase were used as 480,600,400IU/g,respectively. The protein yield extracted by cellulase and Alcalace was 35% higher than that extracted by Alcalace. In the same time,it was 50% higher than that extract by cellulose. The oil holding capacity,emulsifying activity,emulsifying stability,and foaming capacity of protein extracted by enzyme were higher than those of protein extracted by alkaline,but the water holding capacity and foam stability were lower than those of protein extracted by alkaline.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第3期127-130,共4页
Science and Technology of Food Industry
基金
江苏省农业攻关项目(BE2009414)
关键词
茶树修剪叶
蛋白质
提取
酶法
pruned tea leaves
protein
extraction
enzyme
